Dataset Information


Fatb Induction Experiment (FatBIE)

ABSTRACT: This experiment tests the consequence of a mutation at the FatB gene (At1g08510) in the wound-response of Arabidopsis. The FatB mutant allele (fatb KD J. Ohlrogge (Plant Cell 2003, Vol 15, 1020-1033)) was obtained from Dr. Katayonn Dehesh, University of California, Davis, Davis, CA. This allele is in the Ws background.The standardized growth conditions are as follows: 1. Seeds (between 14 and 16) are sown on media in 100 x 100 x 15mm square Falcon Petri Dishes (Fisher Scientific, catalogue #08-757-11A). Seeds were arranged on the plates in a single horizontal line at the 1-cm mark from the top of the plate.2. Each plate contains between 20 and 25-ml of sterile MS media containing 0.1% (w/v) sucrose.3. Prior to sowing, seeds were sterilized by treating for 1 minute at room temperature with a 300-l solution of 50% (v/v) ethanol, this solution was removed and replaced with a 300-l solution consisting of 1% (v/v) Tween 20 (Fischer BioReagents, catalogue #BP33750), and 50% (v/v) bleach solution (Clorox), and incubated at room temperature for 10-minutes. The seeds were then washed with three changes of 0.3-ml of sterile water.


TISSUE(S): Plant

SUBMITTER: Tobias Kind  

PROVIDER: ST000001 | MetabolomicsWorkbench | Tue Jan 15 00:00:00 GMT 2013

REPOSITORIES: MetabolomicsWorkbench

Similar Datasets

2013-05-20 | ST000013 | MetabolomicsWorkbench
1000-01-01 | S-EPMC4256969 | BioStudies
1000-01-01 | S-EPMC6349850 | BioStudies
2019-01-01 | S-EPMC6689618 | BioStudies
2017-01-01 | S-EPMC6296612 | BioStudies
2013-11-22 | E-GEOD-52634 | ArrayExpress
2014-01-01 | S-EPMC5453151 | BioStudies
2014-01-01 | S-EPMC4971884 | BioStudies
1000-01-01 | S-EPMC3943537 | BioStudies
1000-01-01 | S-EPMC5290466 | BioStudies