Metabolomics

Dataset Information

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Primary T Cell Noxa Knockdown (Donor 8)-II


ABSTRACT: Labeling of cells was carried out in triplicate with each sample containing 35e6. Cells were deprived of glucose or glutamine for 1 h then resuspended in 10mM [13C1,2] D-glucose labeled or 4 mM [13C5]-U-glutamine labeled medium, respectively. At this time cells were also activated with 5 ug/ml anti-CD3 and anti-CD28. Cells were labeled (and activated) for 16 h when samples were spun down, and cells were resuspended in 200 ul ice cold methanol. All samples were immediately stored in -80°C.

ORGANISM(S): Human Homo Sapiens

TISSUE(S): T-cells

DISEASE(S): Cancer

SUBMITTER: Maureen Kachman  

PROVIDER: ST000153 | MetabolomicsWorkbench | Fri Feb 13 00:00:00 GMT 2015

REPOSITORIES: MetabolomicsWorkbench

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