Metabolomics

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Muscle Clock knock out mice metabolic changes (iMSBmal1-Exp1)


ABSTRACT: My lab studies the function of the molecular clocks in skeletal muscle. We have an inducible genetic mouse model (C57Bl6 background) in which we knock out the core clock gene, Bmal1, only in adult skeletal muscle after treatment with tamoxifen. We have found that the mice maintain body mass but lose fat mass at 10 weeks after loss of Bmal1. We have done expression profiling on the skeletal muscles and gene expression changes (insulin signaling, CHO metabolism, fat metabolism) suggest significant changes in substrate metabolism. To analyze TCA, CHO metabolites we have collected gastrocnemius muscles from these mice following instructions from Dr. Burant. Mice were anaesthetized with isoflurane, the gastrocnemius muscle dissected and flash frozen with tongs cooled with liquid N2. They have been stored in cryovials in our -80 freezer for 2 months.

ORGANISM(S): Mouse Mus Musculus

TISSUE(S): Muscle

SUBMITTER: Maureen Kachman  

PROVIDER: ST000313 | MetabolomicsWorkbench | Thu Mar 26 00:00:00 GMT 2015

REPOSITORIES: MetabolomicsWorkbench

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