Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Primary metabolites at different points along dog gastrointestinal tract (CSH chromatography)

ABSTRACT: This experiment tests the primary metabolites at four different points along the gastrointestinal tract of a dog. The four points being tested were the duodenum, ileum, colon, and rectum.

ORGANISM(S): Canis Lupus Familiaris Dog

TISSUE(S): Intestine, Rectum

SUBMITTER: Oliver Fiehn

PROVIDER: ST000328 | MetabolomicsWorkbench | Thu Feb 12 00:00:00 GMT 2015

REPOSITORIES: MetabolomicsWorkbench

ACCESS DATA

Json Xml

Similar Datasets

Primary metabolites at different points along dog gastrointestinal tract (HILIC chromatography)

Project description:This experiment tests the primary metabolites at four different points along the gastrointestinal tract of a dog. The four points being tested were the duodenum, ileum, colon, and rectum.

2015-02-27 | ST000327 | MetabolomicsWorkbench

Metabolomics Workbench ST000328 - GNPS - Primary metabolites at different points along dog gastrointestinal tract (CSH chromatography)

2018-08-02 | MSV000082703 | GNPS

Metabolomics Workbench ST000327 - GNPS - Primary metabolites at different points along dog gastrointestinal tract (HILIC chromatography)

2018-08-02 | MSV000082698 | GNPS

Multi-omic biogeography of the gastrointestinal microbiota of a pre-weaned lamb

Project description:Here, we used reverse-phase liquid chromatography-coupled tandem mass spectrometry to study the pre-weaned lamb proteome and metaproteome in ten different gastrointestinal tracts: rumen, reticulum, omasum, abomasum, duodenum, jejunum, ileum, cecum, colon, and rectum.

2017-12-14 | PXD008192 | Pride

lncRNA atlas of inflammatory diseases along the GI tract highlights regulatory mitochondrial metabolic epithelial functions of GATA6-AS1

Project description:Long non-coding RNA (lncRNA) mechanisms in gut inflammation are poorly understood. Tissue-specificity of lncRNAs linked to patient outcomes may direct interventions with fewer off-target effects. Using 693 mucosal samples, we prioritize lncRNAs linked with ulcerative colitis (UC) course and define an atlas of lncRNAs expressed along the gastrointestinal tract dysregulated in celiac duodenum, Crohn Disease ileum, and UC rectum using independent test and validation cohorts.

2023-06-21 | GSE199906 | GEO

Metabolomic profiles along the gastrointestinal tract of the healthy dog

Project description:Introduction: The fecal microbiome is relevant to the health and disease of many species. The importance of the fecal metabolome has more recently been appreciated, but our knowledge of the microbiome and metabolome at other sites along the gastrointestinal tract remains deficient. Objective: To analyze the gastrointestinal microbiome and metabolome of healthy domestic dogs at four anatomical sites. Methods: Samples of the duodenal, ileal, colonic, and rectal contents were collected from six adult dogs after humane euthanasia for an unrelated study. The microbiota were characterized using Illumina sequencing of 16S rRNA genes. The metabolome was characterized by mass spectrometry-based methods. Results: Prevalent phyla throughout the samples were Proteobacteria, Firmicutes, Fusobacteria, and Bacteroidetes, consistent with previous findings in dogs and other species. A total of 530 unique metabolites were detected; 199 of these were identified as previously named compounds, but 141 of them had at least one significantly different site-pair comparison. Noteworthy examples include amino acids, which decreased from the small to large intestine; pyruvate, which was at peak concentrations in the ileum; and several phenol-containing carboxylic acid compounds that increased in the large intestine. Conclusion: The microbiome and metabolome vary significantly at different sites along the canine gastrointestinal tract.

2016-10-17 | ST000495 | MetabolomicsWorkbench

Transcription profiling of Hsd:ICR(CD-1) mouse intestines to study regional variations in ABC transporter expression along the mouse intestinal tract.

Project description:8 week-old male Hsd:ICR(CD-1) mice were fed ad libitum a standard chow (Harlan Teklad diet 2018S) and housed in groups of five. Animals were then divided into 3 pools (n=10) and sacrificed. The small intestine was extracted and divided into three sections, where the first 2-3 cm after the stomach comprised the duodenum, the middle third the jejunum, and the section before the ileo-ceco-colic junction comprised the ileum. Colon was not divided into proximal and distal sections and was treated as a single intestinal section.

2007-12-13 | E-GEOD-849 | biostudies-arrayexpress

Protein turnover rate along the gastrointestinal tract is coordinated by the spatial location and the microbiota

Project description:The gastrointestinal tract is covered by a single layer of epithelial cells that, together with the mucus layers, protect the underlying tissue from bacterial invasion. The epithelium has one of the highest turnover rates in the body, renewing every 4-5 days. Using stable isotope labelling, high-resolution mass spectrometry and computational analysis, we report here a comprehensive dataset of the turnover rate of 3041 and the expression of 5012 intestinal epithelial cell proteins, analyzed under conventional and germ-free conditions across five different segments in mouse intestine. The median protein half-life was shorter in small intestine compared to colon, ranging from 3.5 to 4.2 days. Differences in protein turnover rates along the intestinal tract can be explained by distinct physiological functions and site-specific immune responses between the small and large intestine. Absence of microflora resulted in increased protein half-life by approximately one day.

2020-01-24 | PXD011457 | Pride