Metabolomics

Dataset Information

49

High Resolution orbittrap Mass Spectrometer to measure low abundance isotope enrichment in individual muscle proteins


ABSTRACT: Stable isotope-labeled amino acids have long been used to measure the fractional synthesis rate of proteins, although the mass spectrometry platforms used for such analyses have changed throughout the years. More recently, tandem mass spectrometers such as triple quadrupoles have been accepted as the standard platform for enrichment measurement due to their sensitivity and the enhanced specificity offered by multiple reaction monitoring (MRM) experiments. The limit in the utility of such platforms for enrichment analysis occurs when measuring very low levels of enrichment from small amounts of sample, particularly proteins isolated from two-dimensional gel electrophoresis (2D-GE), where interference from contaminant ions impact the sensitivity of the measurement. We therefore applied a high resolution orbitrap mass spectrometer to the analysis of [ring-13C6]-phenylalanine enrichment in individual muscle proteins isolated with 2D-GE. Comparison of samples analyzed on both platforms revealed that the high resolution MS has significantly improved sensitivity relative to the triple quadrupole MS at very low-level enrichments due to its ability to resolve interferences in the m/z dimension.

INSTRUMENT(S): Orbitrap

TISSUE(S): Muscle

SUBMITTER: Sreekumaran Nair  

PROVIDER: ST000543 | MetabolomicsWorkbench | Fri Jul 22 00:00:00 BST 2016

REPOSITORIES: MetabolomicsWorkbench

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