Dataset Information

Human oral keratinocytes growth and proliferation based on culture medium volume

ABSTRACT: We seeded cells at density of 500k/T75 and cultured them at two different medium volume, one at 30ml, the other at 15ml. Culture medium is composed of 1x EDGS and 0.06mM calcium. We changed medium every day for 30ml culture, and every two days for 15ml culture. We collected spent medium when we changed medium. The collected spent medium was filtered through low protein binding 0.22um pore size filter and then stored at -80 degree C. For OA and SMT, spent medium was collected on the same day of D5 and D9 after cell seeding as marked on the tubes. For GK samples, 15ml and 30ml cultures were collected when cells reached at around 90% confluence, that is D5 for 15ml and D6 for 30 ml culture. The numbers 151, 152 were duplicated cell cultures in two T75s, same for 301 and 302. One major differences among GK, OA, and SMT is GK was from frozen cells; OA and SMT were fresh cells that were never frozen. Blank15 and Blank30 were complete medium without cells incubating in incubator for two days for Blank15 and one day for Blank30. Medium was collected in the same manner as others.

ORGANISM(S): Human Homo Sapiens

TISSUE(S): Keratinocytes

SUBMITTER: Maureen Kachman

PROVIDER: ST000690 | MetabolomicsWorkbench | Mon Jun 19 00:00:00 BST 2017

REPOSITORIES: MetabolomicsWorkbench

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Human oral keratinocytes growth and proliferation based on culture medium volume

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