Metabolomics

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Deubiquitinase inhibitor compound C6 effect on cellular metabolism


ABSTRACT: 6.0 X 10^6 cells were plated in 10 cm Tissue Culture dishes and allowed to recover overnight at 37 degrees and 5% CO2. In the am, supernatant was removed, and 12 mL of fresh medium with either 2.5 micromolar Compound C6 or a v/v match of DMSO (vehicle control) was added to the cells. Cells were incubated at 37 degrees in 5% CO2 for 30 minutes. Cells were washed 3X with cold DPBS++ and 10 mL of medium was added to the cells with either 1.0 micromolar Compound C6 or v/v match of DMSO. Cells were incubated at 37 degrees and 5% CO2 for 7.0 hours. Cells were washed with 10 mL of 150 mM Ammonium Acetate, swirled, washed again with 5 mL, and immediately quenched with liquid nitrogen. Cells were then frozen at -80 degrees.

ORGANISM(S): Mouse Mus Musculus

TISSUE(S): Cultured Cells

SUBMITTER: Maureen Kachman  

PROVIDER: ST000835 | MetabolomicsWorkbench | Wed Aug 02 00:00:00 BST 2017

REPOSITORIES: MetabolomicsWorkbench

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