Project description:Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH-MS) is a DIA method whose use in proteomic studies has increased considerably within the past five years. SWATH-MS acquires a complete and permanent digital record for all the detectable MS/MS spectra of a sample using DIA. After their generation, the SWATH-MS maps can be used for iterative analyses of candidate proteins. As with any analytical methodology that has potential widespread use, several studies have been conducted to optimize and evaluate the performance of SWATH-MS. The present dataset was acquired from 103 tissue samples of high-grade serous ovarian carcinoma collected and processed in the context of the CPTAC initiative and analyzed via bottom-up SWATH mass spectrometry.
Project description:High-grade ovarian cancer (HGSOC) is a highly fatal disease with frequent recurrence and high mortality rates, despite ongoing treatment advancements. Next-generation sequencing (NGS) is an experimental technique that can identify extensive genetic information, making it a key component of precision medicine. We conducted a study based on a sample of 108 patients retrospectively selected from Severance Hospital and Gangnam Severance Hospital. We aimed to identify genetic factors associated with HGSOC recurrence and survival using deep targeted sequencing. This study identified key genetic alterations associated with recurrence and survival in HGSOC and confirmed that somatic mutations are linked to disease prognosis. We expect that these findings will contribute to more precise prognosis prediction and the development of personalized therapeutic strategies for patients with recurrent ovarian cancer.
Project description:To investigate mechanisms involved in the dysplastic progression of SSA, we evaluated differential expressions of mRNAs between areas with and without dysplasia within same SSA polyps.