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Sub-nanoliter metabolomics via mass spectrometry to characterize volume-limited samples - MSC

ABSTRACT: The human metabolome provides a window into the mechanisms and biomarkers of various diseases. However, because of limited availability, many sample types are still difficult to study by metabolomic analyses. Here, we present a new mass spectrometry (MS)-based metabolomics strategy that only consumes sub-nanoliter sample volumes. The approach consists of combining a customized metabolomics workflow with a pulsed MS ion generation method, known as triboelectric nanogenerator inductive nanoelectrospray ionization (TENGi nanoESI) MS. A second example to illustrate TENGi MS capabilities involves rare cell metabolomics of cultured mesenchymal stromal cells (MSCs), a cell type that has shown potential for treating a variety of chronic diseases. Examination of metabolic changes of MSCs cultured under conditions that may impact in vitro therapeutic activity, such as aggregate culture, or preconditioning with interferon gamma (IFN- γ)13, is critical for identifying attributes of cell quality. Reducing cell numbers required to perform MSC metabolomic analysis is essential for improving the manufacturing of highly therapeutic MSCs without significantly impeding production.

ORGANISM(S): Human Homo Sapiens

TISSUE(S): Mesenchymal Stromal Cells

DISEASE(S): Cystic Fibrosis

SUBMITTER: Facundo Fernandez

PROVIDER: ST001438 | MetabolomicsWorkbench | Sun Aug 02 00:00:00 BST 2020

REPOSITORIES: MetabolomicsWorkbench

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