Metabolomics

Dataset Information

0

Glutamine flux in macrophages treated with stable-isotope labeled analog 4 mM (U-13C5) glutamine


ABSTRACT: 107 BMDMs per group (Sirt3 WT and Sirt3 K223R)were seeded in 10cm plates and incubated in RPMI-1640 cell culture medium with 10% FBS. Prior to isotopic labeling, the medium was replaced with RPMI-1640 without glutamine for 4 hrs. Then stable-isotope labeled analog 4 mM (U-13C5) glutamine (Cambridge Isotope) was added together with IL-4 (20ng/ml) for 4 hrs.

ORGANISM(S): Mouse Mus Musculus

TISSUE(S): Macrophages

SUBMITTER: Wei Zhou  

PROVIDER: ST002061 | MetabolomicsWorkbench | Wed Jan 19 00:00:00 GMT 2022

REPOSITORIES: MetabolomicsWorkbench

Similar Datasets

2015-12-31 | E-GEOD-65562 | biostudies-arrayexpress
2012-06-25 | GSE37580 | GEO
2020-01-11 | GSE143418 | GEO
2020-01-28 | GSE144272 | GEO
2012-06-24 | E-GEOD-37580 | biostudies-arrayexpress
2019-03-29 | PXD012053 | Pride
2014-10-01 | E-MTAB-1684 | biostudies-arrayexpress
2003-04-16 | GSE404 | GEO
2010-10-05 | E-GEOD-19779 | biostudies-arrayexpress
2016-02-17 | GSE69923 | GEO