Project description:Changes in cellular metabolism contribute to the development and progression of tumors, and can render tumors vulnerable to interventions. However, studies of human cancer metabolism remain limited due to technical challenges of detecting and quantifying small molecules, the highly interconnected nature of metabolic pathways, and the lack of designated tools to analyze and integrate metabolomics with other âomics data. Our study generates the largest comprehensive metabolomics dataset on a single cancer type, and provides a significant advance in integration of metabolomics with sequencing data. Our results highlight the massive re-organization of cellular metabolism as tumors progress and acquire more aggressive features. The results of our work are made available through an interactive public data portal for cancer research community. 10 RNA samples from human ccRCC tumors analyzed from the high glutathione cluster
Project description:ACSS2 is a key contributor to the acetyl-CoA pool and has a role in supporting biosynthetic processes, as well as acetylation. To investigate the role of ACSS2 in regulating chromatin accessibility in ccRCC, we performed ATAC-seq on 786-O cells treated with DMSO or ACSS2i for 24 hours.