Project description:Hepatocellular carcinoma samples were obtained after patients provided written informed consent and with the approval of the institutional research ethics board at the Liver Cancer Institute of Zhongshan Hospital, Fudan University (Shanghai, China). Fresh tumor tissues were implanted in NOD/SCID mice. Tumor lines were successfully maintained through multiple rounds of serial transplantation. We used microarrays to evaluate the genotype similarity between patient tumor and patient-derived xenograft (PDX).
Project description:Paraffin-embedded paired tumor and peri-carcinoma tissues (n=4) were obtained from the Biorepository of Fudan University Institute of Liver Cancer, which were used for the Affymetrix microarray analysis To investigate the progression of NASH to HCC, we established a differential gene expression profile by analyzing significant expression changes in pathologically confirmed NASH-HCC and peri-carcinoma specimens using Affymetrix microarrays.
Project description:The purpose of this study is to analysis 41 RNA modification enzymes in 1659 HBM samples from 10 public datasets, and 100 HCC samples from Zhongshan Hospital of Fudan University (Shanghai, China). we designed an RH score model to predict the clinical prognosis, response to molecular targeted drugs and immunotherapy and transcriptional and posttranscriptional events, thereby providing a novel panel of next-generation sequencing for clinical translation.
Project description:Hepatocellular carcinoma samples were obtained after patients provided written informed consent and with the approval of the institutional research ethics board at the Liver Cancer Institute of Zhongshan Hospital, Fudan University (Shanghai, China). Fresh tumor tissues were implanted in NOD/SCID mice. Tumor lines were successfully maintained through multiple rounds of serial transplantation. We used microarrays to evaluate the expression similarity between patient tumor and patient-derived xenograft (PDX).
Project description:Human Ovarian tissue samples including 15 normal tissue samples and 48 malignant ovarian tissue were collected at Zhongshan Hospital of Fudan University. All tissue samples were immediately frozen in liquid nitrogen after being removed from body and stored at -80°C. Biovue's Sharpvue miRNA qPCR array panels (A-E) including 1721 unique assays were used to quantitate genome-wide miRNA gene expression from the collected ovarian tissue samples.
Project description:In this study, we collected 10 normal oral mucosa samples and 10 OLP samples separately from Huashan Hospital, Fudan University. This study was approved by the Institutional Research Ethics Committee of Huashan Hospital, Fudan University (Approval No.KY2019-589, KY2022-1000), and every patient has signed an informed consent form.
Project description:1Sheng Yushou Center of Cell Biology and Immunology, Department of Genetics and Developmental Biology, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China. 2Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, NY 10065, USA. 3Systems Biology Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA. 4CCTS Bioinformatic Program, The Rockefeller University, New York, NY 10065, USA. 5State Key Laboratory of Genetic Engineering & Ministry of Education Key Laboratory of Contemporary Anthropology, Collaborative Innovation Center of Genetics and Development, School of Life Sciences, Fudan University, Shanghai, 200438, China
Project description:Raw data of TMT-labeled proteome of Chinese breast cancers. Uploaded by Zhi-Ming Shao Lab, Fudan University Shanghai Cancer Center (FUSCC)
Project description:This is a single blind, case control, multicenter study jointly developed by Zhongshan Hospital of Fudan University, Shanghai Public Health Clinical Center, Shanghai Xuhui Central Hospital, Qingpu Branch of Zhongshan Hospital Affiliated to Fudan University, and Shanghai Singlera Genomics Company. The enrolled population will include positive group, precancerous lesions and healthy control group, which is expected to enroll 2,430 participants. The primary objective is to establish molecular testing methods for non-invasive screening and early diagnosis of digestive system cancers through ctDNA methylation and mutation, cfDNA and ctDNA fragment size, and end motif based model (for esophageal, gastric, colorectal cancer), and through ctDNA methylation detection, ctDNA low-pass WGS, miRNA7 and CTC detection and analysis technology based model (for hepatocellular carcinoma). The sensitivity and specificity of the models in cancer early detection will be evaluated.