Project description:We investigated the contribution of NOX-2 to redox balance, mitochondrial respiration, and glycolytic compensation in lineage-negative Sca-1⁺c-Kit⁺ (SKL) bone marrow stem cells from wild-type (WT) and NOX-2 knockout (NOX2-KO) mice. Proteomic profiling identified differentially expressed proteins involved in hypoxic response, glycolysis, and oxidative stress adaptation between WT and NOX2-KO SKL cells.
Project description:NSUN2, a C-type lectin receptor, has been implicated in macrophage-mediated immunity, but its role in M1 polarization remains unclear. Here, we performed RNA sequencing on bone marrow-derived macrophages (BMMs) from NSUN2-knockout (KO) and wild-type (NC) mice following M1 stimulation.
Project description:ATAC-seq profiling of Nfat5 KO and wild type macrophages derived from bone marrow (primary cells), treated or not with Lipopolysaccharide (LPS).
Project description:To fully elucidate the effects of long non-coding RNA 5730403I07Rik (lnc57Rik) and lncGM1082 on myeloid-derived suppressor cell (MDSCs), we generated lnc57Rik knockout (KO) and lncGM1082 ko mice. Then bone marrow cells were obtained from the femurs of C57BL/6, lnc57Rik KO, or lncGM1082 ko mice and cultured in RPMI-1640 medium supplemented with GM-CSF plus IL6 for 4 days. We further performed a high-throughput sequencing analysis in cultured MDSCs obtained from bone marrow of lnc57Rik ko, lncGM1082 ko and wild type (WT) mice.
Project description:LSKs(lineage-, Sca-1+, c-kit+ cells) in bone marrow from wild type (WT) and MC5R-knockout (KO) mice after after irradiation were isolated to explore the differentially expressed genes by RNA-seq.
Project description:To investigate the role of METTL3-mediated m6A modification in macrophage, we performed RNA-sequencing in bone-marrow-derived macrophages (BMDMs) in wild type (WT) and Mettl3-/- (KO) mice We then performed gene expression profiling analysis using data obtained from RNA-seq of WT and KO BMDMs
Project description:RNA transcriptome difference between WT and SIRPa knockout (KO) bone marrow derived macrophages (BMDMs). To understand how SIRPa inhibits the phagocytotic capacity of macrophages, a mouse lacking SIRPa was generated, and the transcriptional profiles of BMDMs from wild-type and SIRPa KO mice were compared, using RNA sequencing.
Project description:LSKs (lineage-, Sca-1+, c-kit+ cells) in the bone marrow from wild type (WT) and Tespa1-knockout (KO) mice at day 13 after 5.0 Gy irradiation were isolated to explore the differentially expressed genes by RNA-seq.
Project description:Bone marrow cells were isolated from wild type and Tbx21 knockout mice. iNK (Lin- NK1.1+ CD11b- CD27+) and mNK1 (Lin- NK1.1+ CD11b+ CD27+) were sorted from WT and Tbx21 KO mice. mNK2 (Lin- NK1.1+ CD11b+ CD27-) were sorted from WT mice only, since these cells are not present in Tbx21 KO mice.