Project description:ATAC-seq was performed in the ovarian cancer cell lines COV-434, ES-2, Kuramochi, OAW28, OAW42, OVISE, SKOV-3, TOV-112D. The aim of this experiment was to detect open chromatin sites. Enhancers are associated with open chromatin sites flanked by histones with H3K4me1 and H3K27ac marks. Therefore, the combination of this ATAC-seq experiment with histone ChIP-seq is complementary for the detection of enhancers. Omni-ATAC-seq was performed as described by Corces et al. 2017 with some modifications, which are described in detail in the protocol.
Project description:Assess chromatin accessibility in WT and mutant ES E14 strains using ATAC-seq. Mutant strains are depleted for subunits of the coactivator complexes SAGA (Supt7l KO) or ATAC (Yeats2, Zzz3) using constitutive knock-out (KO) or the auxin-inducible degron (AID) system.
Project description:To determine the effect of Zscan10 on chromatin accessibility, mouse ES cells (mESCs) carrying Zscan10 gene endogenously tagged with an FKBP12(F36V) degron were treated with dTAGv-1 to induce Zscan10 degradation and were assayed with ATAC-seq.