Nematode metagenome
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REPOSITORIES: ENA
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Project description:Once Haemonchus contortus infects sheep it receives a series of host attacks, especially those relating to the infected animal’s T lymphocytes immune response. To obtain a systematic genome-wide profiling of the T lymphocyte genes involved, microarrays were used to compare gene expression between 0 days post infection (dpi), 3-5 dpi, 25-30 dpi and 60dpi in infected sheep. In this 853, 242 and 42 differentially expressed genes were acquired in the 3d vs. 0d comparison, the 30d vs. 0d comparison and the 60d vs. 0d comparison, respectively. Gene Ontology and pathway analysis indicated that modulated genes including SUGT1, FCER1G, CD23, IL-13 and galectin-14, were mostly associated with cellular homeostasis maintaining and immune response. Haemonchus contortus infection induced gene expression in sheep T lymphocytes was measured at 0, 3, 30 and 60 days post infection. Four time-series experiments were performed at each sheep (2#, 3# and 5#).
2012-11-16 | E-GEOD-42302 | biostudies-arrayexpress
Project description:Illumina sequencing of small RNAs from Brugia pahangi and Haemonchus contortus
2011-12-20 | GSE34539 | GEO
Project description:Examined expression of microRNAs in different life-cycle stages and gut tissue of the pathogenic nematode Haemonchus contortus and identified differentially expressed miRNAs. Custom array contained probes to 609 Haemonchus contortus miRNA sequence (all mature and start miRNAs identified by Winter et al., 2012, BMC Genomics13:4; DOI: 10.1186/1471-2164-13-4) and all C. elegans sequences in miRBase release 16
2017-11-07 | GSE101501 | GEO
Project description:The Alpine goat Capra aegagrus hircus is parasitized by the barber pole worm (Haemonchus contortus). This relationship results in changes that affect the gene expression of the host, the pest, and the microbiome of both. Hematological parameters indicating genes that are expressed and/or the % Composition of abundant and diverse microbial flora are reflective of infestation. We identified responses to barber pole worms using blood-based analysis of transcripts and the microbiome. Seven (7) days post-inoculation (dpi) we identified 7,627 genes associated with different treatment types.
2021-03-26 | GSE169607 | GEO
Project description:Illumina sequencing of small RNAs from Brugia pahangi and Haemonchus contortus 4 samples examined, larval stage 3 and mixed sex adults from two parasitic nematode species.
2011-12-20 | E-GEOD-34539 | biostudies-arrayexpress
Project description:N-glycosylation is a physiologically vital post-translational modification of proteins in eukaryotic organisms. Initial work on Haemonchus contortus – an economically-important blood-sucking nematode of ruminants with a broad geographical distribution – has shown that the parasite harbors N-glycans with exclusive core chitobiose modifications, and severel immunogenic proteins (e.g., amino- and metallo-peptidases) in the adult worms are N-glycosylated. However, an informative atlas of N-glycosylation in H. contortus is not yet available. Herein, we report a total of 291 N-glycosylated proteins with 425 sites in this parasite. Functional analyses of glycoproteome revealed significant enrichment of the peptidase families (e.g., peptidase C1 and M1), many of which are potential vaccine targets. Besides, the glycan-rich conjugates are distributed primarily in the intestine and gonads of the adult worms. These data, taken together, provide a comprehensive insight into the N-glycosylation of a prevalent parasitic nematode, while underlining its significance for the infection and prophylaxis.
2021-09-09 | PXD023379 | Pride
Project description:Once Haemonchus contortus infects sheep it receives a series of host attacks, especially those relating to the infected animal’s T lymphocytes immune response. To obtain a systematic genome-wide profiling of the T lymphocyte genes involved, microarrays were used to compare gene expression between 0 days post infection (dpi), 3-5 dpi, 25-30 dpi and 60dpi in infected sheep. In this 853, 242 and 42 differentially expressed genes were acquired in the 3d vs. 0d comparison, the 30d vs. 0d comparison and the 60d vs. 0d comparison, respectively. Gene Ontology and pathway analysis indicated that modulated genes including SUGT1, FCER1G, CD23, IL-13 and galectin-14, were mostly associated with cellular homeostasis maintaining and immune response.
2012-11-16 | GSE42302 | GEO
Project description:Parasitic worms have a major global impact in human and animal populations due to the chronicity of their infections. These parasites have an exquisite capacity to survive in host animals. There is a growing body of evidence that extracellular vesicles (EVs) are intimately involved in parasitism, and that these vesicles are instrumental in modulating (suppressing) inflammatory/immune responses. The recent expansion of proteomic and lipidomic technologies and molecular resources for some parasitic worms (order Strongylida) provide a unique opportunity to conduct profound (qualitative and quantitative) explorations of EVs, with the prospect of being able to elucidate the functional roles of key molecules within these vesicles in host tissues and cells. As one of the most pathogenic nematodes of livestock animals, the blood-feeding barber’s pole worm – Haemonchus contortus – is an ideal model system for EV exploration. Here, using advanced methods, we defined the proteome of EVs of Haemonchus contortus. We identified and quantified 561 proteins in these EVs, and compared these molecules with those of adult worms. We identified unique molecules in EVs such as proteins linked to lipid transportation and lipid species (i.e. glycerophospholipids and sphingolipids) associated with signalling, indicating an involvement of these molecules in parasite-host cross-talk. This work provides a solid starting point to explore the functional roles of EV-specific proteins and lipids in modulating parasite-host cross talk; a foundation to assess the utility of such molecules as biomarkers for the specific detection of infection or disease; and the prospect of finding ways to disrupt or interrupt this relationship to suppress or eliminate infection.
2023-08-07 | PXD041918 | Pride