Dataset Information

N-Glycome and N-Glycoproteome of a Hematophagous Parasitic Nematode Haemonchus

ABSTRACT: N-glycosylation is a physiologically vital post-translational modification of proteins in eukaryotic organisms. Initial work on Haemonchus contortus – an economically-important blood-sucking nematode of ruminants with a broad geographical distribution – has shown that the parasite harbors N-glycans with exclusive core chitobiose modifications, and severel immunogenic proteins (e.g., amino- and metallo-peptidases) in the adult worms are N-glycosylated. However, an informative atlas of N-glycosylation in H. contortus is not yet available. Herein, we report a total of 291 N-glycosylated proteins with 425 sites in this parasite. Functional analyses of glycoproteome revealed significant enrichment of the peptidase families (e.g., peptidase C1 and M1), many of which are potential vaccine targets. Besides, the glycan-rich conjugates are distributed primarily in the intestine and gonads of the adult worms. These data, taken together, provide a comprehensive insight into the N-glycosylation of a prevalent parasitic nematode, while underlining its significance for the infection and prophylaxis.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Haemonchus Contortus (barber Pole Worm)

TISSUE(S): Whole Body

SUBMITTER: Chunqun Wang

LAB HEAD: Min Hu

PROVIDER: PXD023379 | Pride | 2021-09-09

REPOSITORIES: Pride

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Publications

N-glycome and N-glycoproteome of a hematophagous parasitic nematode Haemonchus.

Wang Chunqun C Gao Wenjie W Yan Shi S Zhu Xing-Quan XQ Suo Xun X Liu Xin X Gupta Nishith N Hu Min M

Computational and structural biotechnology journal 20210418

N-glycosylation is a physiologically vital post-translational modification of proteins in eukaryotic organisms. Initial work on Haemonchus contortus - a blood-sucking nematode of ruminants with a broad geographical distribution - has shown that this parasite harbors N-glycans with exclusive chitobiose modifications. Besides, several immunogenic proteins (e.g., amino- and metallo-peptidases) are known to be N-glycosylated in adult worms. However, an informative atlas of N-glycosylat ...[more]

PMID: 34025939

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Dataset Information

N-Glycome and N-Glycoproteome of a Hematophagous Parasitic Nematode Haemonchus

Publications

N-glycome and N-glycoproteome of a hematophagous parasitic nematode <i>Haemonchus</i>.

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