Project description:Transformation of Glycine max with seed-targeted expression vectors via Agrobacterium causes measurable unscripted gene expression changes in the seed transcriptome
Project description:The diurnal transcriptome of field-grown Glycine max was investigated in relation to diurnal physiological processes in the field and compared to diurnal transcription data from growth chamber studies
Project description:Glycine max was cultivated in China for nearly 5000 years, commonly referred to as soybeans, now it has become one of the important economic crops in the world (Li et al., 2008). Post-translational modifications are known to regulate many cellular processes, which are dynamic and reversible and can make protein functions changed.(Westermann and Weber, 2003). To date, among 400 PTMs have been detected, such as Acetylation, Ubiquitination, Phosphorylation, Malonylation, Succinylation and Methylation(Colak et al., 2013; Weinert et al., 2013).
Project description:Cytosine methylation is a base modification that is often used by genomes to store information that is stably inherited through mitotic cell divisions. Most cytosine DNA methylation is stable throughout different cell types or by exposure to different environmental conditions in plant genomes. Here, we profile the epigenomes of ~100 Glycine max lines to explore the extent of natural epigenomic variation. We also use these data to determine the extent to which DNA methylation variants are linked to genetic variations.
Project description:Tropospheric ozone (O3) is a secondary air pollutant and anthropogenic greenhouse gas. Concentrations of tropospheric O3 have more than doubled since the Industrial Revolution, and are high enough to damage plant productivity. Soybean (Glycine max L. Merr.) is the worldâs most important legume crop and is sensitive to O3. Current ground-level O3 are estimated to reduce global soybean yields by 6% to 16%. In order to understand transcriptional mechanisms of yield loss in soybean, we examined the transcriptome of soybean flower and pod tissues exposed to elevated O3 using RNA-Sequencing.
Project description:Little is known about plant pathogenic response to parasitic plants, although some parasitic plants affect crop production in certain areas. To study this, we chose Glycine max as the model host plant and investigated changes in expression patterns after parasitization by Cuscuta using microarrays. Transcriptional change of Glycine max stem with and without Cuscuta at 2 different stages were compared
Project description:We report here a transcriptonal analysis in six different organ types of a approximately 1 Mb region in soybean (Glycine max) which is sytenic with legume (Medicago truncatula). We used oligonucleotide tiling microarrays to detecte transcription of over 80% of the predicted genes in both species. We detected differential gene expression in the six examined organ types. Keywords: RNA
Project description:Transcript profiling of control vs Mungbean yellow mosaic India virus infected Glycine max variety JS335. RNA samples were collected at 2 dpi to study change in transcript profile at early infection.
