Project description:We found that several deacetylase-dead HDAC3 mutants were able to rescue the metabolic phenotype of HDAC3-depleted livers. Here we profile the histone acetylation in the presence of different HDAC3 mutants in mouse liver. Deacetylase-dead HDAC3 mutants, including HAHA, KA, YF and HEBI, were introduced into HDAC3-depleted (Cre) mouse livers by virus along with wild-type (WT) HDAC3 as a control. Livers were harvested at 5 pm (ZT 10) and subjected to ChIP with anti-H3K9ac antibodies followed by deep sequencing.
Project description:To confirm epithelial gene expression of the large intestine after inoculation of dead (heat-treatment) or live Bifidobacterium probiotic strain (BbrY) we have employed whole genome microarray expression profiling. The epithelial cells were released from dead or live BbrY associated mice 3 or 28 days after association and GF mice with HANKS including EDTA and Hepes and treated by TRI-zol reagent. It was confirmed that the live BbrY associated mice were affected epithelial gene expression much more than dead cell feeding by K-means clustering and the functional categories.
Project description:Small RNAs were cloned from wildtype and Ago2 catalytic dead mouse fetal liver to uncover slicing dependent miRNAs in hematopoietic tissue
Project description:DNGR-1 (CLEC9A) is expressed in CD8-like dendritic cells (DCs) and detects a ligand exposed on necrotic cells. We have characterized the transcripts that are induced in cultures of Flt3L-derived bone-marrow-derived WT or DNGR1-deficient DCs upon incubation with dead cells. Microarray analysis of the transcriptome of DNGR-1-deficient and WT DCs cultured with dead cells failed to reveal any DNGR-1-dependence in the transcriptional response to dead cells. Flt3L-BMDC from WT or DNGR-1-deficient (Clec9a gfp/gfp) mice were left untreated or were cultured for 5 hours with UV-treated dead cells at a 1:2 ratio. CD24hi CD11blow B220- CD8α+-like DC were purified by cell sorting and RNA was extracted. There were 3 replicates for each of the four experimental conditions.
Project description:Induction of the expression of the DHH1 DEAD:DQAD mutant from pLEW100 for 24 hours (thus, M-1 24 hours <br>Tetracycline). Uninduced cells were used as control.
