Project description:Reproducibility assessment is essential in extracting reliable scientific insights from high-throughput experiments. Inconsistency between technical replicates poses a challenge, particularly evident in NGS technologies based on immunoprecipitations, where the need for reproducibility in peak identification is a well-acknowledged limitation. While the Irreproducibility Discovery Rate (IDR) method has been instrumental in assessing reproducibility, its standard implementation is constrained to handling only two replicates. In the current era of steadly growing sample sizes, facilitated by multiplexing and reduced sequencing costs, highly performing methods that handle any number of replicates are desirable.
Project description:We used microarrays to assess the reproducibility of global gene expression in rat lung from experiments performated by 2 individuals. Expression analyses in 01matsu and 01suzu rat lung. Both prepared under the same conditions, and the comparison is between the reproducibility of the experiment as performed by two different individuals.
Project description:Five aliquots of the same RNA sample were labeled and hybridized in parallel to the L. vannamei microarray, in order to assess the technical reproducibility of the tool Keywords: technical validation
Project description:We used microarrays to assess the reproducibility of global gene expression in rat lung from experiments performated by 2 individuals. Expression analyses in 01matsu and 01suzu rat lung. Both prepared under the same conditions, and the comparison is between the reproducibility of the experiment as performed by two different individuals. Affymetrix® GeneChip® Rat Genome 230 2.0 Array was used to analyze total RNA from rat lung.
Project description:The purpose of this work was to describe a computational and analytical methodology for profiling small RNA by high-throughput sequencing. The datasets here were used to assess the reproducibility of small RNA datasets produced using Illumina sequencing-by-synthesis technology (SBS).