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Expression data from Ribonuclease Inhibitor (RI/Rnh1) deficient Human K562 cells

ABSTRACT: Expression data from Ribonuclease Inhibitor (RI/Rnh1) deficient Human K562 cells

PROVIDER: PRJEB19635 | ENA |

REPOSITORIES: ENA

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Expression data from Ribonuclease Inhibitor (RI/Rnh1) deficient Human K562 cells

Project description:Summary: Ribonuclease Inhibitor (RI also known as Rnh1) is a 50 kDa, ubiquitously expressed leucine-rich repeat (LRR) protein. It is localized in cytosol and binds to pancreatic-type ribonucleases and inhibit their function. However, the entire biological role for Rnh1 is unknown. We generated RNH1 knock out K562 cells by CRISPR/Cas9 method. Here we studied differential gene expression from wild type and RNH1 knock out K562 cells by RNA-Seq analysis. Overall design: Total RNA was isolated from wild type and RNH1 deficient K562 cells.

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Expression data from Ribonuclease Inhibitor (RI/Rnh1) deficient E9.5 yolksac

Project description:Ribonuclease Inhibitor (RI also known as Rnh1) is a 50 kDa, ubiquitously expressed leucine-rich repeat (LRR) protein. It is localized in cytosol and binds to pancreatic-type ribonucleases and inhibit their function. However the entire biological role for Rnh1 is unknown. We generated Rnh1 knock out mice by homologous recombination. Here we studied differential gene expression from wild type (Rnh1 +/+), Heterozygous (Rnh1+/-) and Knock out (Rnh1-/-) yolk sacs isolated from embryonic day 9.5 (E9.5). We used microarrays to study global gene expression regulated by Rnh1 in yolk sacs.

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Expression data from Ribonuclease Inhibitor (RI/Rnh1) deficient E9.5 yolksac

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Project description:Expression data from Ribonuclease Inhibitor (RI/Rnh1) deficient Human K562 cells

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Regulation of mRNA translation by RNH1 in hematopoietic cells

Project description:Ribonuclease Inhibitor (RI also known as Rnh1) is a 50 kDa, ubiquitously expressed leucine-rich repeat (LRR) containing protein. It binds to pancreatic-type ribonucleases and inhibit their function. However, the entire biological role of Rnh1 is unknown. We generated RNH1 knock out K562 cells by CRISPR/Cas9 method. We isolated polysomal RNA from control and RNH1-deficient K562 cells to quantify actively translated mRNAs by RNA-seq.

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