RNAseq of Quinoa salt bladders vs. leaves
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ABSTRACT:
PROVIDER: PRJEB22942 | ENA |
REPOSITORIES: ENA
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Project description:Chenopodium quinoa, a pseudo-cereal and facultative halophyte, is a species of great economic potential. When exposed to saline soil, this salt-tolerant crop takes up sodium and chloride ions and sequesters large NaCl quantities in epidermal bladders cells (EBC). We have analyzed the Quinoa EBC transcriptome by RNA sequencing and elucidated the molecular identity and function of key ion transporters. Thereby we analyzed transcripts differentially expressed between EBCs and total leaves under control conditions.
2018-09-20 | E-MTAB-6112 | biostudies-arrayexpress
Project description:Quinoa, like a large fraction of halophytes, use so-called bladder cells to detoxify excess salt. These leaf exterior structures are formed by specialized trichomes and consist of a leaf epidermal cell, a stalk cell and the bladder cell itself. Under salt stress, Na+ and Cl- as well as K+ and metabolites are imported from leaf sources and stored in the bladder. During this process the stalk cell simultaneously operates as both a selectivity filter and a flux controller. We submerged detached leaves in buffer, then lightly brushed the abaxial surface with a fine paintbrush to remove only the EBCs. The abaxial epidermis was then removed using tweezers and sampled in buffer for RNA extraction and RNA sequencing to separate the gene expression profiles of stalk cells from bladders and leaves.
2022-04-01 | E-MTAB-10363 | biostudies-arrayexpress
Project description:Quinoa (Chenopodium quinoa) is a highly nutritious crop showing a remarkable tolerance to multiple abiotic stresses. The Catharanthus roseus RLK1-like kinase (CrRLK1L) family is involved in multiple processes during plant growth and stress responses. However, little is known about the members of the CqCrRLK1L subfamily and their biological functions. In this study, up to 26 CqCrRLK1L members were identified in quinoa genome. To systematically investigate their tissue-specific expression pattern and gene expression profiles in response to abiotic stresses, different quinoa samples including leaf, pistil, stamen, root, and leaf treated with NaCl were harvested for transcrioptome analysis with three biological replicates for each sample. Finally,we mapped about 30 million sequence reads per sample to the quinoa genome (NL-6) and combining the four different tissues (leaf, pistil, stamen, and root), totally 40382 genes were detected, accounting for 69% of total genes in quinoa. The expression of most CqCrRLK1Ls genes were detected in all four kind of tissues, and they showed tissue-specific expression pattern. In salt-treated leaves, we observe that about 6-thosands genes changed their expression, with log2FoldChange < -1 or >1 and p value <0.05. And four CqCrRLK1L genes largely altered their transcript levels, implying that these genes are involved in the regulation of salt stress response in quinoa. The present study provides a base for future research on elucidating the varied biological functions of CqCrRLK1Ls and their contributions to stress responses.
2022-06-27 | GSE198572 | GEO
Project description:We generated 70.9 Gb of high-quality sequencing data (~7.88 Gb per sample) and catalogued the expression profiles of 54，238 annotated Chenopodium quinoa genes in each sample. These genes have known or potential roles in the roots, stems, and leaves of quinoa. Therefore, we are appealing candidates for further investigation of the gene expression and associated regulatory mechanisms.
2022-03-03 | GSE156523 | GEO
Project description:The experiments were used to detect proteins in ethylene regulated salt response in quinoa. The quinoa is one of healthy food source for human. Ethylene is a stress hormone, which improves salt tolerance in plants by regulating adaptive changes in gene expression level. Proteomic analysis provides an efficient method to excavate downstream functional genes in a large scale. In order to clarify the ethylene regulated salt response pathway in quinoa, the whole seedlings of 4-week-old quinoa treated with water, salt, salt and ethylene precuorsor ACC, respectively, and analyzed by proteomic analysis in this research.
2021-05-23 | PXD026210 | iProX
Project description:Quinoa is a highly nutritious seudocereal crop that is not well adapted to heat. Improving understanding of quinoa responses to heat would help expand its cultivation to regions with warmer climates. This study profiled gene expression of quinoa plants from accession QQ74 (PI 614886) treated with high tempreatures during anthesis of the main panicle, which lasted 11 days. Heat was applied in four different treatments to profile gene expression changes from root or shoot heating. Treatments were: 1. control with both roots and shoots at 22°C, 2. heated roots with roots at 30°C and shoots at 22°C, 3. heated shoots with roots at 22 °C and shoots at 35°C, and 4. heated roots and shoots with roots at 30°C and shoots at 35°C. Healthy leaves from 3 plants per treatment were sampled from the mid section of the plants, during the first (day 1) and last day (11) of heat treatment, at Zeitgeber time 2. Gene expression of each of the three heat treatments was compared to control using the Kallisto-Sleuth pipeline. These gene expression profiles of quinoa under heat are made available as a community resource to improve understanding of quinoa responses to heat.
2019-07-31 | GSE128155 | GEO
Project description:In this project, leaves and EBCs of the halophyte plant Chenopodium quinoa were treated with ABA. The effect of the ABA treatment was analyzed by comparing to non-treated leaves and EBCs.
2022-05-10 | E-MTAB-10419 | biostudies-arrayexpress
Project description:Grapevine rootstock 1616C shoots were sterilized and cultured on Murashige & Skoog (MS) medium containing 2% sucrose (w/v). Plantlets were grown in a growth chamber with a 16-h light/8-h dark cycle for 10 weeks at 25 °C. Plantlets with 4–5 leaves were chosen for use in stress treatments. Experiments were conducted with treatment groups: The control (C, without any chemical treatment), TM (treated with 5 μg mL-1 tunicamycin (TM)) and salt (treated with 400 mM NaCl). Microarray analysis was performed and we investigated transcript profiles in leaves of the salt-tolerant grapevine rootstock 1616C under salt- and ER-stress at 6 and 24 hours
2020-07-10 | GSE150581 | GEO
Project description:We generated 95.37 Gb of high-quality sequencing data (~7.95 Gb per sample). The analysis showed differences of transcriptomes between the common white sweet quinoa and the yellow bitter quinoa. We identified numerous differentially expressed genes that exhibited distinct expression patterns. These genes have known or potential roles in taste of quinoa fruit.Therefore, we are appealing candidates for further investigation of the gene expression and associated regulatory mechanisms related to the accumulation of bitter saponins in C. quinoa fruits.
2021-09-17 | GSE139174 | GEO
Project description:Transcriptome profiling of Chenopodium quinoa in response to salt
| PRJNA636120 | ENA