Project description:Transcriptional profiling of Arabidopsis buds comparing ahl16 mutants with wild type (Col-0 ecotype). Goal was to determine the differential expression genes between the buds of mutant and wild type.
Project description:rs12-08_cyp715a1 - col-0 vs cyp715a1 - The microarray analysis is part of a project aimed at characterizing the function of the cytochrome P450 CYP715A1 in Arabidopsis thaliana. - Flower buds of Arabidopsis Col-0 (wild-type) and cyp715A1 mutant were harvested for a comparative analysis of their transcriptomes.
Project description:Transcriptional profiling of Arabidopsis buds comparing ahl16 mutants with wild type (Col-0 ecotype). Goal was to determine the differential expression genes between the buds of mutant and wild type. Biological replicates: 3 replicates of mutant buds, 3 replicates of wildtype buds.
Project description:Tomato flowering and fruit set require an optimal temperature of 25/22 ± 2˚C (day/night). When the air temperature reaches to above the optimal range (higher than 30/26˚C; day/night), only a small number of flower buds would develop into mature flowers and produce a reduced number of pollen. This project used the iodoTMT proteomics analysis method to identify heat-induced proteomes in these tomato flower buds.
Project description:rs12-08_cyp715a1 - col-0 vs cyp715a1 - The microarray analysis is part of a project aimed at characterizing the function of the cytochrome P450 CYP715A1 in Arabidopsis thaliana. - Flower buds of Arabidopsis Col-0 (wild-type) and cyp715A1 mutant were harvested for a comparative analysis of their transcriptomes. 3 dye-swap - gene knock out,genotype comparaison
Project description:MAGLs participate lipid mobilization and endocannabinoid signalling in mammal. However, their potential biological function in plants remains elusive. In a survey of BnMAGL molecular function, BnA9::BnMAGL8.1 transgenic plants were outstanding with their male sterile phenotype. To discover the mechanism undering the phenotype, we conducted RNAseq on flower buds in stage 6-9 from BnA9::BnMAGL8.1 transgenic plants with wild type (Col ecotype).
Project description:Gene expression profile of flower buds at stage 13, open flowers at stage 14 and siliques at stages 15/16, according to Smyth et al., 1990) of ABAP1 overexpressing (ABAP1OE) plants compared to wild type flower buds, open flowers and siliques (Col-0) using a a whole-genome oligonucleotide array (Operon) (platform accession number accession number GPL1077). ABAP1 is a negative regulator of the cell cycle that binds to transcription factors and represses their target gene expression, including pre-replication complex genes. All experiments were performed in triplicate.
Project description:Col-0 floral stem was grafted on the msh1 mutant (Col-0/msh1); on the dcl2,3,4,msh1 quadruple mutant (Col-0/dcl2,3,4,msh1); on Col-0 (Col-0/Col-0). Seeds were collected from the grafted Col-0 scion after grafts were established. Seed coming from the graft then were grown on the peat mix, leaf tissue was collected at the bolting and used for the total RNA sequencing.
Project description:Transcriptional profiling of Arabidopsis buds comparing dyt1, tdf1, ams, ms188 mutants with wild type (Col ecotype), respectively. Goal was to determine the differential expression genes between the buds of mutant and wild type.