Project description:Effect of a dietary supplement with antioxidant and anti-inflamatory properties on the intestinal microbiota in patients with colon cancer. Ramdonized placebo controlled clinical trial. Teratrophic study
Project description:Patients with early stage CLL were recruited into the study and administered a course of omega 3 dietary supplements. Expression profiling of each patient was performed at baseline (before administering the supplement) and after consumption of supplements. Patients were divided into two groups according to the level of NFkB activation at baseline, and then within each of the two groups significant changes in gene expression between baseline and the highest dose of supplements consumed were sought. Patients were divided into two groups based on initial NFkB levels being below (low expressers) or above (high expressers) the median NFkB Activity level (2.14x10^6 NFkB Luminescence units/µg protein). Within each group, paired two class SAM was performed using the maximum supplement dose for each patient versus the baseline.
Project description:Patients with early stage CLL were recruited into the study and administered a course of omega 3 dietary supplements. Expression profiling of each patient was performed at baseline (before administering the supplement) and after consumption of supplements. Patients were divided into two groups according to the level of NFkB activation at baseline, and then within each of the two groups significant changes in gene expression between baseline and the highest dose of supplements consumed were sought.
Project description:In this study, we investigated the effects of organic vegetable juice supplementation on modulating the microbial community, and how its consumption ameliorates blood lipid profiles in diet-induced obese mice. Here, we analyzed the effect of organic vegetable juice on the microbial community and fatty acid synthesis via animal experiments using diet-induced obese mice and continuous colon simulation system. Organic vegetable juice supplement influenced intestinal bacterial composition from phylum to genus level, including decreased Proteobacteria in the ascending colon in the phylum. At the family level, Akkermansia which are associated with obesity, were significantly augmented in the transverse colon and descending colon compared to the control juice group. In addition, treatment with organic vegetable juice affected predicted lipid metabolism function genes related to lipid synthesis. Organic vegetable juice consumption did not have a significant effect on weight loss but helped reduce epididymis fat tissue and adipocytes. Additionally, blood lipid profiles, such as triglyceride, high-density lipoprotein, and glucose, were improved in the organic vegetable juice-fed group. Expression levels of genes related to lipid synthesis, including SREBP-1, PPARγ, C/EBPα, and Fas, were significantly decreased. Analysis of antioxidant markers, including 8-OHdG and MDA, in the vegetable juice group, indicated that blood lipid profiles were improved by the antioxidant effect. These results suggest that organic vegetable juice supplementation may modulate gut microbial community and reduce the potential role of hyperlipidemia in diet-obese mice.
Project description:Origanum oil (ORO), garlic oil (GAO), and peppermint oil (PEO) were shown to effectively lower methane production, decrease abundance of methanogens, and change abundances of several bacterial populations important to feed digestion in vitro. In this study, the impact of these essential oils (EOs, at 0.50 g/L), on the rumen bacterial community composition was further examined using the recently developed RumenBactArray.
Project description:This experiment aims to analyze spent media from a protein over-expression system. The treatment was a lipid supplement given to hamsters. The spent media was then analyzed to see how the lipid supplement affected lipid metabolism.
