Solitary bee dysbiosis
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB38245 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:During the nest-founding phase of the bumble bee colony cycle, queens undergo striking changes in maternal care behavior. Early in the founding phase, prior to the emergence of workers in the nest, queens are reproductive and also provision and feed their offspring. However, later in the founding phase, queens cease feeding offspring and become specialized on reproduction. This transition is synchronized with the emergence of workers in the colony, who assume the task of feeding their siblings. Using a social manipulation experiment, we tested the hypothesis that workers socially regulate the transition from feeding brood to specialization on reproduction in nest-founding bumble bee queens. Consistent with this hypothesis, we found that early-stage queens with workers prematurely added to their nests reduce their brood-feeding behavior and increase egg-laying, and likewise, late-stage queens increase their brood-feeding behavior and decrease egg-laying when workers are removed from their nests. Further, brood-feeding and egg-laying behavior were negatively correlated in these queens. We used an Agilent brain EST-based microarray to explore a second hypothesis, that workers alter brain gene expression in nest-founding queens. We found evidence that brain gene expression in nest-founding queens is altered by the presence of workers, with the effect much stronger in late-stage founding queens. Additionally, expression levels of some genes were correlated with quantitative differences in brood-feeding and egg-laying behavior. This study provides new insights into how the transition from feeding brood to specialization on reproduction in bumble bee queens is regulated during the nest initiation phase of the colony cycle.
2014-06-21 | E-MTAB-1855 | biostudies-arrayexpress
Project description:Linking pollen foraging of megachilid bees to their nest bacterial microbiota
| PRJEB31610 | ENA
Project description:We studied the molecular mechanisms underlying the impact of pollen nutrients on honey bee (Apis mellifera) health and how those nutrients improve resistance to parasites. Using digital gene expression, we determined the changes in gene expression induced by pollen intake in worker bees parasitized or not by the mites Varroa destructor, known for suppressing immunity and decreasing lifespan of bees.
2011-11-06 | GSE25161 | GEO
Project description:Microbiome of stingless bee nest Raw sequence reads
| PRJNA715737 | ENA
Project description:Individual differences in behaviour are often consistent across time and contexts, but it is not clear if such consistency is reflected at the molecular level. We explored this issue by studying scouting in honey bees in two different behavioural and ecological contexts: finding new sources of floral food resources and finding a new nest site. Brain gene expression profiles in food-source and nest-site scouts showed a mixture of similarities and differences. Class prediction and 'leave-one-out' cross-validation analyses revealed that a bees role as a scout in either context could be predicted with 92.5% success using 89 genes at minimum. We also found that genes related to four neurotransmitter systems were part of a shared brain molecular signature in both types of scouts, but the two types of scouts were more similar for genes related to glutamate and GABA and more divergent for genes related to monoamines and acetylcholine signaling. These results indicate that consistent behavioural tendencies across different ecological contexts involve a mixture of similarities and differences in brain gene expression.
2014-10-31 | E-MTAB-2918 | biostudies-arrayexpress
Project description: Not available
| S-EPMC5537383 | biostudies-other
Project description:Apis mellifera syriaca is the native honeybee subspecies of Jordan and much of the Middle East. It expresses behavioral adaptations to a regional climate with very high temperatures, nectar dearth in summer, attacks of the Oriental wasp Vespa orientalis and in most cases it is resistant to varroa mites. The Thorax control sample of A. m. syriaca in this experiment was originally collected and stored since 2001 from Wadi Ben Hammad a remote valley in the southern region of Jordan. Using morphometric and Mitochondrial DNA markers it was proved that bees from this area had show higher similarity than other samples collected from the Middle East as represented by reference samples collected in 1952 by Brother Adam. The samples L1-L5 are collected from the National Center for Agricultural Research and Extension breading apiary which was originally established for the conservation of Apis mellifera syriaca. Goal was to use the genetic information in the breeding for varroa resistant bees and to determine the successfulness of this conservation program. Project funded by USAID-MERC grant number: TA-MOU-09-M29-075.
2016-02-01 | GSE43554 | GEO
Project description:Honey bee embryos were injected either with a CRISPR contruct targeting the orco gene or an injection buffer control. RNA-Sequencing was performed on the antennal mRNA from adult bees within 24 hours of eclosion.
2020-10-09 | GSE147713 | GEO
Project description:Honey bee embryos were injected either with a CRISPR contruct targeting the orco gene or an injection buffer control. RNA-Sequencing was performed on the antennal mRNA from adult bees within 24 hours of eclosion.
2020-10-09 | GSE147712 | GEO