Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Small RNA-seq of Arabidopsis pol2a mutants

ABSTRACT: small RNA-seq of Arabidopsis pol2a mutants

PROVIDER: PRJEB38702 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

small RNA-seq of Physcomitrella patens small RNA mutants

Project description:To investigate the effects of dcl3,dcl4,mDCL,rdr2,rdr6,nrpd1b, and nrpd1c mutations upon small RNAs in Physcomitrella patens , small RNA-seq was performed. Total RNA samples were obtained from the indicated mutants and subject to small RNA-seq using the Illumina TruSeq Small RNA protocol, and analyzed on a HiSeq2500 instrument

2015-06-30 | E-GEOD-51419 | biostudies-arrayexpress

Small RNA biogenesis mutants

Project description:Inflorescence stages 1 to 12 from mutants involved in Arabidopsis small RNA metabolism. Three biological replicates of each mutant comprising at least 9 independent plants were harvested, and the expression profiles were determined using Affymetrix ATH1 arrays. Comparisons among the sample groups allow the identification of genes regulated by small RNAs (microRNAs and siRNAs). Keywords: other

2005-05-09 | GSE2473 | GEO

High-throughput pyrosequencing of endogenous small RNAs from Arabidopsis thaliana wild-type and polIV mutants

Project description:Comparison of the endogenous small RNA content of Arabidopsis flower bud tissue: wild type vs. mutants in polIV pathways Keywords: High throughput 454 small RNA sequencing.

2008-02-25 | GSE10180 | GEO

Analysis of small RNA population in Arabidopsis drb2 and drb4 mutants

Project description:We have compared populations of small (21 to 24-nt) RNAs from Arabidopsis immature flowers of WT, drb2 and drb4 mutants and found that DRB2 and DRB4 are needed for the proper biogenesis of polymerase IV-dependent siRNAs Examination of small RNA populations from three backgournd: Col0 (WT), drb2 and drb4 mutants

2012-03-01 | E-GEOD-27517 | biostudies-arrayexpress

Analysis of small RNA population in Arabidopsis drb2 and drb4 mutants

2012-03-01 | GSE27517 | GEO

RNA-seq and small RNA-seq in fission yeast CPF mutants that enable small-RNA-mediated epigenetic gene silencing

Project description:RNA-seq and small RNA-seq in fission yeast CPF mutants that enable small-RNA-mediated epigenetic gene silencing

| PRJNA727318 | ENA

small RNA-seq of Arabidopsis pol2a mutants

Project description:DNA replication requires the faithful propagation of both genetic and epigenetic information. There is evidence that DNA polymerases play a role in transcriptional silencing, but the extent of their contribution and how it relates to heterochromatin maintenance is unclear. Analyzing a new hypomorphic pol2a mutant allele, we find that POL2A, the catalytic subunit of the DNA polymerase epsilon, maintains heterochromatin silencing and nuclear organization. We also found that POL2 inhibits DNA methylation and histone H3 lysine 9 methylation, which both correlate with 24-nucleotide small RNA accumulation. In this experiment, we sequenced small-RNA libraries generated from immature inflorescences of pol2a-10 and pol2a-12 mutants and wild-type plants.

2020-06-04 | E-MTAB-8938 | biostudies-arrayexpress

Dicer-independent RNA-directed DNA methylation in Arabidopsis [small RNA-seq 2]

Project description:Using small RNA-Seq(sRNA-Seq) to get small RNA profiling of dcl2/3/4/nrpd1 and nrpd1 mutants

2016-03-17 | GSE71672 | GEO

Small RNA deep sequencing of Arabidopsis DRB mutants

Project description:Arabidopsis thaliana (Arabidopsis) encodes five DOUBLE-STRANDED RNA BINDING (DRB) proteins, DRB1 to DRB5, that predominantly act as non-catalytic cofactors for DICER-LIKE (DCL) proteins in the double-stranded RNA (dsRNA) processing stages of small RNA (sRNA) production pathways. In the nucleus, DRB1 is required for microRNA (miRNAs) processing from imperfectly dsRNA precursors by DCL1. Similarly, DRB4 is required by DCL4 for small-interfering RNAs (siRNAs) production from endogenous or exogenous perfectly dsRNA templates. DRB2 has been recently demonstrated to be required for miRNA and siRNA production in developmentally-important tissues of Arabidopsis while the requirement of either DRB3 or DRB5 in sRNA production remains unclear. Here, we analyse in parallel, the contribution of all five DRB protein family members to the global sRNA landscape of Arabidopsis floral tissues. In depth bioinformatic analysis of sRNA sequencing datasets generated from floral tissues of DRB knockout mutant (drb) plant lines, drb1, drb2, drb4, drb12, drb14, drb24, and drb35 and their comparison to the floral sRNA profile of wild-type Arabidopsis, has enabled confident assignment of the requirement of DRB1, DRB2 and DRB4 for the production of specific miRNA and siRNA subclasses in this tissue. Our analyses have additionally identified novel and/or expanded roles for DRB2 in miRNA, trans-acting siRNAs (tasiRNAs) and natural antisense transcript siRNAs (natsiRNAs) production.

2017-09-09 | GSE73752 | GEO

Transcription profiling of Arabidopsis inflorescence stages 1-12 from small RNA biogenesis mutants

2008-06-12 | E-GEOD-2473 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data