Project description:A number of studies have reported evidence of positive or negative contributions of galectin-9 (gal-9) to human and experimental malignancies. Some clinical observations and in vitro experiments suggest that cell-associated gal-9 has anti-metastatic effects. On the other hand, extra-cellular gal-9 consistently enhances tumor immune escape. So far, all animal studies on this subject have been focused on gal-9 released by infiltrating cells, without paying attention to gal-9 released by malignant cells. To address this issue, we derived by gene editing, isogenic clones - either positive or negative for gal-9 - from the MB49 murine bladder carcinoma cell line. A progressive reduction of tumor growth was observed when gal-9-KO cells were subjected to serial transplantations into syngenic mice but not into nude mice thus accounting the tumor growth reduction in syngenic mice to a better immune response. Tumor fragments were collected from WT and KO tumors at different steps of the experiment : 2nd growth cycle (WT = 5 samples ; KO = 6 samples ) ; 3rd growth cycle (WT = 5 samples ; KO = 7 samples) ; 4th growth cycle (WT = 4 samples ; KO = 3 samples), in order to study the differences between WT and KO tumors through the serial transplantations, by RNAseq analysis.

2020-07-01 | E-MTAB-9220 | biostudies-arrayexpress

Exome-Seq of murine MB49 cell line - WT and gal-9-KO - in vitro

Project description:Exome-Seq of murine MB49 cell line - WT and gal-9-KO - in vitro

| PRJEB41649 | ENA

RNAseq of murine CT26 cell line -WT and gal-9-KO - in vitro

2020-09-20 | E-MTAB-9559 | biostudies-arrayexpress

RNAseq of murine CT26 cell line -WT and gal-9-KO - in vitro

Project description:RNAseq of murine CT26 cell line -WT and gal-9-KO - in vitro

| PRJEB40308 | ENA

Exome-Seq of murine CT26 cell line - WT and gal-9-KO - in vitro

2021-01-01 | E-MTAB-9837 | biostudies-arrayexpress

RNAseq of murine MB49 tumors - WT and gal-9-KO - grown on C57BL/6 syngenic mice through serial transplantations

Project description:RNAseq of murine MB49 tumors - WT and gal-9-KO - grown on C57BL/6 syngenic mice through serial transplantations

| PRJEB38913 | ENA

Exome-Seq of murine CT26 cell line - WT and gal-9-KO - in vitro

Project description:Exome-Seq of murine CT26 cell line - WT and gal-9-KO - in vitro

| PRJEB41648 | ENA

RNAseq of MB49 inoculated tumor-assotiated macrophages from MC-proficient and MC-deficient animals

Project description:RNA seq was used to compare the expression profile of macrophages in presence and absense of mast cells. MB49 cells were injected i.d. into Mcpt5-Cre+ R26DTA animals and cre-negative littermates. Macrophages were sorted at 20 d.p.i.

2017-12-13 | GSE101995 | GEO

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