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RNA-seq of human U87MG-GFP perivascular glioma stem cells

ABSTRACT: RNA-seq of human U87MG-GFP perivascular glioma stem cells

PROVIDER: PRJEB40521 | ENA |

REPOSITORIES: ENA

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RNA-seq of human U87MG-GFP perivascular glioma stem cells

Project description:Glioblastoma stem cells (GSCs) have been reported to be found near the blood vessels. We wanted to identify the perivascular GSCs by combining the label retention approach with vascular perfusion. GSCs, like most of the stem cells, are in a quiescent state. Label retention approach involves staining the cells with a uniform membrane labeling dye (PKH26) and allowing them to grow in vivo in NOD SCID mice. Slow-dividing stem cells will retain the dye to a greater extent and perivascular cells are identified by injecting the mice with vascular perfusion dye (Hoechst 33342) 5 min prior to sacrifice. Perivascular GSCs (positive for Hoechst 33342 and pKH26 dye - dubbed as H+P+) were compared with the rest of the perivascular glioma cells (H+P-).

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Differential gene expression analysis between adherent U87MG cells and U87MG cell-derived glioma stem-like cells (P4E8 cells).

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Silencing of ASCL1 in U87MG glioma cells

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Gene expression analysis of the human glioma cell line U87MG

Project description:Identification of genes and pathways regulated by kynurenine in human glioma cells Total RNA from U87MG cells was extraced after 8h or 24h of treatment with 100µM kynurenine and compared to untreated control.

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Differential gene expression analysis between adherent U87MG cells and U87MG cell-derived glioma stem-like cells (P4E8 cells).

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Differential gene expression analysis of U87MG EGFRvA cells vs. U87MG EGFR cells

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Human glioma cells: Control (GFP) vs PDGF-A expressing

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Established glioma cell lines prove valuable models for tumour heterogeneity - mediated cellular response studies in vitro

Project description:Both established glioma cells lines U87MG and U373 were used for studying their interactions in the indirect co-cultures. Eventhough both being derived from the glioma tissue, those two cell lines prove morphologically and physiologically disctinct. Therefore their intre-cellular interactions were examined on gene exprexssion level in vitro to observe whether those co-cultures could make for a suitable in vitro cell model mimicking the in vivo glioma tumour heterogeneity. Three biological replicates of four cell set-ups were performed: U87MG monoculture, U373 monoculture, U87MG co-cultured with U373 (in Boyden chambers) and U373 co-cultured with U87MG (in Boyden chambers).

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