Project description:Primary objectives: The primary objective is to investigate circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
Primary endpoints: circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
Project description:Eight tissues of cultivar Morex (three biological replications each) earmarking stages of the barley life cycle from germinating grain to maturing caryopsis were selected for deep RNA sequencing (RNA-seq)
Project description:Chevallier is a heritage english landrace of barley first planted in 1820 while Tipple is modern cultivar of barley released in 2004. Pseudomonas strains were isolated from the rhizospheres of the two varieties and 22 and 20 of the most phylogenetically distinct ones were sequenced to find out the difference in genotypes preferentially selected in the rhizospheres of the two cultivars.
Project description:Ustilago hordei is a basidiomycete fungus that infects barley and oats. It has a narrow host range and can infect only germinating seedlings. Symptoms do not occur until heading of the mature plants when the fungus has proliferated in meristematic tissue and replaces kernels with masses of black teliospores. Incompatibility is due to the presence of an avirulence effector product, UhAVR1, expressed in the fungus upon infection and secreted into the host, which is subsequently recognized by the barley plant resistance gene product RUH1 in cultivar Hannchen, leading to plant cell death, fungal growth arrest, and no disease. We have identified UhAvr1, and the transcriptome response of the barley plant to strains with and deleted for this gene was assessed using the 22K Affymetrix barley array. Incompatibility and cell death is apparent at 48 hrs after inoculation, so the transcriptome was assessed at this time point. For comparison, the transcriptome of barley cultivar Odessa, which lacks resistance gene Ruh1, during a compatible interaction was also assessed.
