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Near-infrared fluorescent reporter stem cell model

ABSTRACT: Near-infrared fluorescent reporter stem cell model

PROVIDER: PRJEB51223 | ENA |

REPOSITORIES: ENA

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Gene expression in a 3-dimensional human epidermal tissue culture model after water-filtered near-infrared irradiation

Project description:To clarify the effects of near-infrared radiation, we assessed DNA microarray after water-filtered near-infrared (1100-1800 nm together with a water-filter that excludes wavelengths 1400-1500 nm) irradiation.

2016-03-19 | GSE79394 | GEO

Gene expression in a 3-dimensional reconstructed human corneal epithelial tissue after near-infrared irradiation simulating solar near-infrared radiation that reaches human tissues on the Earthâs surface

Project description:To clarify the effects of near-infrared radiation, we assessed DNA microarray after water-filtered broad-spectrum near-infrared (1100-1800 nm together with a water-filter that excludes wavelengths 1400-1500 nm) irradiation. We performed 5 rounds of near-infrared irradiation (at 10 J âcm2) using 2 sets of transparent polycarbonate plates, one to block UV and the other to block both UV and near-infrared.

2016-01-05 | E-GEOD-76500 | biostudies-arrayexpress

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A multiple reporter protein tolerant mouse model

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mCherry contains a fluorescent protein isoform that interferes with its reporter function

Project description:Fluorescent proteins are essential reporters in cell biology and molecular biology. Here, we reveal that red-fluorescent proteins possess an alternative translation initiation site that produces a short functional protein isoform. The short isoform creates significant background fluorescence that biases the outcome of expression studies. Our investigation identifies the short protein isoform, traces its origin, and determines the extent of the issue within the family of red fluorescent protein. Our analysis shows that the short isoform defect of the red fluorescent protein family may affect the interpretation of many published studies. Finally, we provide a re-engineered mCherry variant that lacks background expression as an improved tool for imaging and protein expression studies.

2022-04-19 | PXD032954 | Pride

Identification of dormant cells using a novel fluorescent reporter of RNA-polymerase II activity

Project description:Dormancy is a reversible cellular state in which the cell cycle and metabolic activity of a cell is dramatically decreased, leading to protection from genetic damage and prolonged survival. One example of dormant cells is the somatic stem cell, which is crucial for tissue homeostasis and response to injury or transplantation. However, while dormant cells have been well characterized in some tissues, the ability to identify them irrespective of tissue of origin remains elusive. Moreover, in some tissues (e.g. the intestinal epithelium) dormant stem cells showed heterogeneity in the marker’s profile that makes their identification and isolation challenging. Here, we developed a live cell reporter of dormancy based on our observation that phosphorylation of RNA Polymerase II (RNApII), a hallmark of active mRNA transcription elongation, is largely absent in dormant stem cells from multiple lineages. We show that insertion of a short RNApII kinase target peptide directly into the yellow fluorescent protein Venus generates a live cell Optical Stem Cell Activity Reporter (OSCAR) for detection of dormant cells. We found, using small intestinal crypt cultures, that OSCAR is able to show the dynamics of dormancy induction and cellular differentiation in real time. Generation and analysis of mice ubiquitously expressing OSCAR showed several populations of OSCARhigh and OSCARlow cells. Microscopy, RNA-Seq and single-cell culture confirmed that OSCAR enables direct identification and isolation of cell populations bearing different transcriptional states and provides a useful tool for understanding dormant cell biology.

2021-04-16 | GSE147030 | GEO

Application of Near Infrared-Indocyanine Green Imaging System in Robot/Laparoscopic Colorectal Cancer Surgery: A Single Center, Prospective Controlled Trial

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