Project description:Somatic embryogenesis closely resembles zygotic embryogenesis and hence, it is considered as a model system to explore dynamic events of embryogenesis, at a molecular level. We sequenced three district developmental time points of somatic embryo development in Arabidopsis thaliana with the aim of exploring transcriptomes at a global scale.
Project description:In this project, we profiled the dynamics of proteome during Arabidopsis early embryogenesis using nanoproteomics. In addition, we identified some proteins which may be important during this process. Combining with RNA-seq, we unveiled the relationship between RNA and proteins during Arabidopsis early embryogenesis.
Project description:Using nanoproteomics,we profiled the dynamics of proteome and found potentially important proteins in Arabidopsis thaliana early embryogenesis. Combining with RNAs-seq, we unveiled the relationship of protein and mRNA during Arabidopsis embryogenesis.
Project description:In order to study Traf6 function during early zebrafish embryogenesis, transcriptome expression profiling was performed at 30% epiboly using a morpholino based knock-down approach.
Project description:The microarray test aims to find the miRNAs involved in somatic embryogenesis in Arabidopsis. The plant microRNA array V2.0 (CapitalBio Corp., Beijing, China) contained 426 non-reduplicated plant miRNA probes, including 188 in Arabidopsis. Finally, 75 plant miRNAs expressed differentially, 36 increased and 39 decreased included. Two critical period samples of somatic embryogenesis were chosen for the microarray test: the edge of embryonic calli in embryonic callus-inducing medium (ECIM) for 14 days and the secondary somatic embryos protuberances in somatic embryo-inducing medium (SEIM) for 2 days (marked as “14D” and “J2D” accordingly) . Three chip were test in each sample.
Project description:We collected tissues from bent cotyledon stage zygotic embryos, proliferating tissue at day 7 and day 14 induction of somatic embryogenesis and mature somatic emrbyos in a wild type (Col-0) and vtc2 (SALK_146824) insertion. We used microarrays to identify global patterns of gene activity during somatic embryogenesis in a wild type (Col-0) and vitamin C deficient mutant (vtc2) RNA was extracted and amplified from four stages of somatic embryogenesis (bent cotyledon, day 7 induction, day 14 induction, and mature somatic embryos) in a wild type (Col-0) and vitamin C deficient mutant (vtc2) before being hybridized to the Arabidopsis ATH1 GeneChip in duplicate (two biological replicates).
