Project description:LongSAGE library in this series are from 'Whole Genome Analysis of Pathogen-Host Recognition and Subsequent Responses in the Rice Blast Patho-System' project. This work is supported by NSF-PGRP #0115642. Keywords: other
Project description:With the availability of a high quality draft rice genome sequence, large mutant collections, and gene expression oligo arrays for rice, we are now well positioned to dissect rice defense pathways. To do this, we performed global expression analyses to identify genes that are differentially expressed in 10 mutant lines (i.e., Xa21, NH1ox, NRR1ox, GR978, spl11, spl17, NBS2-PI9ox, MPK5ox, OsCoi1ox, and OsNahG1ox), exhibiting altered defense responses. As controls, we used wild typle varieties same with these mutants. Keywords: disease response
Project description:LongSAGE library in this series are from 'Whole Genome Analysis of Pathogen-Host Recognition and Subsequent Responses in the Rice Blast Patho-System' project. This work is supported by NSF-PGRP #0115642. Keywords: other
Project description:Whole genome arrays have been used to analyze the transcriptomic response to vanadium stress in rice root. Identify genes and pathways that would respond to vanadium stress
Project description:Transcription factor encoded by OsbZIP39 gene is regulator of the Endoplasmic Reticulum Stress Response. The truncated form of OsbZIP39 without the transmembrane domain (OsbZIP39deltaC) is an active form. To identify the potential downstream genes regulated by OsbZIP39, we performed the rice 44k oligo microarray analysis.
Project description:With the availability of a high quality draft rice genome sequence, large mutant collections, and gene expression oligo arrays for rice, we are now well positioned to dissect rice defense pathways. To do this, we performed global expression analyses to identify genes that are differentially expressed in 10 mutant lines (i.e., Xa21, NH1ox, NRR1ox, GR978, spl11, spl17, NBS2-PI9ox, MPK5ox, OsCoi1ox, and OsNahG1ox), exhibiting altered defense responses. As controls, we used wild typle varieties same with these mutants. Two-condition experiment, 10 mutants vs wild type control with treatment or without treatment. Biological replicates: 2-4 control, independently grown and harvested. Technical replicates: 1-2 control. One replicate per array.
Project description:In this study, we used RNA-Seq to understand the mechanisms of Cd toxicity, cellular detoxification and protection pathways in response to Cd in rice roots. To gain additional insight into the rice transcriptomic response to environmental Cd stress, 15-day-old rice seedlings were treated with 10 or 100 μM solutions of Cd2+, or without Cd (control), for 24 h, at which point root samples were harvested and labeled as Cd+, Cd++, and control, respectively. These samples were used for 101 bp paired-end (PE) deep sequencing on an Illumina HiSeq 2500 platform.
Project description:Transcription factor encoded by OsbZIP39 gene is regulator of the Endoplasmic Reticulum Stress Response. The truncated form of OsbZIP39 without the transmembrane domain (OsbZIP39deltaC) is an active form. To identify the potential downstream genes regulated by OsbZIP39, we performed the rice 44k oligo microarray analysis. Total RNA was extracted from 7-day-old rice shoots of the wild type control and the OsbZIP39deltaC overexpression line grown on the MS medium, and subjected to 44k oligo-DNA microarray with 4 biological replicates.
Project description:Rice blast disease caused by Magnaporthe oryzae is one of the most damaging diseases affecting rice productivity. Previously, we reported a novel M. oryzae- secreted protein MSP1, which triggers cell death and pathogen-associated molecular pattern (PAMP)-triggered immune (PTI) responses in rice. To investigate the MSP1 induced defense response in rice at the protein level, we employed a label-free quantitative proteomic approach, in parallel with the flg22, which is a wellknown elicitor. Proteomics analysis using the MaxQuant-Perseus platform led to the identification of 4087 proteins of which 417 showed significant differences (multiple sample test, ANOVA p<0.05) in response to MSP1 and/or flg22 treatments. Functional annotation of the differential proteins showed that proteins related to the primary metabolism, secondary metabolism and lipid metabolism were strongly down-regulated, while elevated proteins were mainly associated with the stress response, chromatin remodeling, post-translational modification of proteins and signaling.
Project description:In this study, we analyzed the early response of two rice cultivars to infection by RSV (Rice stripe virus) and its carrier at the transcriptome level using next-generation deep-sequencing techniques. We investigated the alteration in gene expression between a disease-resistant cultivar and a susceptible cultivar before and after inoculation with RSV by co-culturing with Laodelphax striatellus for 48 h. Our study provides insight at the molecular level into the mechanism of development of rice stripe disease, which contributes to our understanding of the rice-RSV interaction.