Project description:E2F4 wild and knockout type neurospheres from E12.5 forebrain. E2F4 transcription factor regulates the expression of the genes which are required for neural stem cell expansion in the developing mouse brain. The results of microarray analysis will help to identify the signaling pathways affected by E2F4 deletion and refine the neural stem cell regulatory mechanism. Keywords: other
Project description:E2F4 wild and knockout type neurospheres from E12.5 forebrain. E2F4 transcription factor regulates the expression of the genes which are required for neural stem cell expansion in the developing mouse brain. The results of microarray analysis will help to identify the signaling pathways affected by E2F4 deletion and refine the neural stem cell regulatory mechanism. Experiment Overall Design: This experiment include 2 samples on 2 platforms for a total of 4 Samples.
Project description:Neural precursor cells from the ganglionic eminence at E14.5 were isolated and cultured as neurospheres. E2F3 and E2F4 genomic binding sites are mapped by ChIP-on-Chip on wild type or mutant cells.
