Drosophila melanogaster
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA109203 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:This SuperSeries is composed of the following subset Series: GSE11311: Drosophila Sex Hierarchy Regulated Gene Expression in 48 hour APF Pupae GSE11313: Drosophila Gene Expression During Metamorphosis in Wild Type and Germline Minus Pupae Keywords: SuperSeries Refer to individual Series
2009-02-10 | E-GEOD-11316 | biostudies-arrayexpress
Project description:Drosophila melanogaster undergoes a complete metamorphosis, during which time the larval male and female forms transition into sexually dimorphic, reproductive adult forms. To understand this complex morphogenetic process at a molecular-genetic level, we performed whole genome microarray analyses. Genes were identified that were expressed during metamorphosis in both somatic and germline tissues of males and females. Additionally, genes were identified that display sex-specific differences in abundance in both of these tissues at discrete times during metamorphosis. Keywords: time course; wild type; genetic modification; Gene expression was examined at five time points during metamorphosis: 0, 24, 48, 71, and 96 hr After Puparium Formation (APF). Gene expression was examined separately in males and females for both wild type pupae and tudor (tud) progeny. tud progeny have genetically ablated germline tissues. All samples were labeled with Cy5 and compared against a common reference sample labeled with Cy3. The reference sample contained male and female wild type pupae from all stages of metamorphosis. All experiments were conducted in triplicate.
2009-02-10 | E-GEOD-11313 | biostudies-arrayexpress
Project description:Drosophila melanogaster undergoes a complete metamorphosis, during which time the larval male and female forms transition into sexually dimorphic, reproductive adult forms. To understand this complex morphogenetic process at a molecular-genetic level, we performed whole genome microarray analyses. Genes were identified that were expressed during metamorphosis in both somatic and germline tissues of males and females. Additionally, genes were identified that display sex-specific differences in abundance in both of these tissues at discrete times during metamorphosis. Keywords: time course; wild type; genetic modification;
2009-02-11 | GSE11313 | GEO
Project description:To investigate transcriptional effects caused by a severe defect in endo-siRNA production, a gene expression profiling was performed on Drosophila dicer-2 pupae compared to wild-type.
2012-05-21 | GSE35945 | GEO
Project description:The process of metamorphosis relies on choreography of temporal and spatial cues that mediate, in which inappropriate activation of many of these genes results in growth defects, developmental delay and death. Using RNA interference, we have unraveled an essential role for the insect specific dopamine 1(D1)-like receptor Dop1R2 at the late larval/prepupal stage. We used microarrays to evaluate differentially expressed genes during the early/pale pupal stage and identified up-regulation of families of functionally related genes, including ecdysone induced genes, stress and defense/immune response genes, and those that underlie tissue morphogenesis. Drosophila Dop1R2 RNAi or w1118 control early/pale pupae were collected from crosses maintained at 29C. These tissues were processed for hybridization on Affymetrix microarrays (DrosGenome1). Results were used to assess differential expression of genes in Dop1R2 RNAi verses control flies.
2015-09-01 | E-GEOD-66496 | biostudies-arrayexpress
Project description:hemocyte minus versus hemocyte plus drosophila embryos
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Project description:Environmental and facility conditions promote singular gravity responses of transcriptome during Drosophila metamorphosis
| PRJNA154351 | ENA
Project description:Little is known about broad patterns of variation and evolution of gene expression during any developmental process. Here we investigate variation in genome-wide gene expression among Drosophila simulans, Drosophila yakuba and four strains of Drosophila melanogaster during a major developmental transition-the start of metamorphosis. Differences in gene activity between these lineages follow a phylogenetic pattern, and 27% of all of the genes in these genomes differ in their developmental gene expression between at least two strains or species. We identify, on a gene-by-gene basis, the evolutionary forces that shape this variation and show that, both within the transcriptional network that controls metamorphosis and across the whole genome, the expression changes of transcription factor genes are relatively stable, whereas those of their downstream targets are more likely to have evolved. Our results demonstrate extensive evolution of developmental gene expression among closely related species. Keywords: other
2003-03-05 | GSE327 | GEO
Project description:Little is known about broad patterns of variation and evolution of gene expression during any developmental process. Here we investigate variation in genome-wide gene expression among Drosophila simulans, Drosophila yakuba and four strains of Drosophila melanogaster during a major developmental transition-the start of metamorphosis. Differences in gene activity between these lineages follow a phylogenetic pattern, and 27% of all of the genes in these genomes differ in their developmental gene expression between at least two strains or species. We identify, on a gene-by-gene basis, the evolutionary forces that shape this variation and show that, both within the transcriptional network that controls metamorphosis and across the whole genome, the expression changes of transcription factor genes are relatively stable, whereas those of their downstream targets are more likely to have evolved. Our results demonstrate extensive evolution of developmental gene expression among closely related species.
2003-03-04 | E-GEOD-327 | biostudies-arrayexpress
Project description:Drosophila melanogaster undergoes a complete metamorphosis, during which time the larval male and female forms transition into sexually dimorphic, reproductive adult forms. To understand this complex morphogenetic process at a molecular-genetic level, we performed whole genome microarray analyses. In somatic tissues at 48 hour After Puparium Formation (APF), 173 sex-biased transcripts that likely function downstream of the doublesex (dsx) branch of the sex determination hierarchy were identified. The mode of regulation of the sex-specific isoforms of DSX (DSX-F and DSX-M) was examined. It was determined that for most downstream targets, DSX-F and DSX-M regulate gene expression in the same manner, but that one isoform acts as a more potent regulator. Keywords: wild type; genetic modification All microarrays were dual channel with direct comparisons of male versus female or wild type versus mutant. All samples consist of whole body pupae collected at 48 hour After Puparium Formation (APF). For each experiment, four biological replicates were analyzed in a dye-swap design.
2009-02-10 | E-GEOD-11311 | biostudies-arrayexpress