Rattus norvegicus
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ABSTRACT:
PROVIDER: PRJNA109459 | ENA |
REPOSITORIES: ENA
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Project description:Gamma knife surgery (GKS) is used for treatment of various brain disorders. The effects of gamma irradiation to targeted and un-targeted regions were evaluated by monitoring gene expression changes in the unilateral irradiated (60 Gy) and contralateral un-irradiated striata in the rat. Striata of irradiated and control brains were dissected 16 h post-irradiation for analysis by rat whole genome 44K DNA oligo microarray. Results revealed 230 induced and 144 repressed genes in the irradiated striatum and 432 induced and 239 repressed genes in the un-irradiated striatum. The number of altered genes in un-irradiated striatum was more than that in irradiated striatum. Results of RT-PCR and western analyses suggested that gamma-irradiation caused cellular damage, including oxidative stress, in both striata of both hemispheres. Our present results indicate that unilateral irradiation during GKS produce bilateral effects as early as 16 h, the time-period analyzed, and these molecular changes in the un-irradiated striatum are ample proof.
2008-10-18 | GSE13220 | GEO
Project description:To obtain and analyze early retinal changes at molecular level at 24h after ipsilateral intraorbital nerve radiation injury by gamma knife surgery Unilateral intraorbital optic nerves of three Rhesus Macaques were treated by gamma knife surgery (GKS) with irradiated doses of 15Gy, while the contralateral optic nerves and retinas served as the control. Gene expression profiles of control and affected retinas were analyzed with Affymetrix Rhesus Macaque Genome arrays at 24h after treatment
2011-09-29 | E-GEOD-29901 | biostudies-arrayexpress
Project description:To obtain and analyze early retinal changes at molecular level at 24h after ipsilateral intraorbital nerve radiation injury by gamma knife surgery
2011-09-30 | GSE29901 | GEO
Project description:A set of changes is identified in the transcription profile associated with the long-term, but not the acute, response to radiation exposure. The study was performed in vivo using zebrafish. To study the long-term response, 24 hour post-fertilization embryos were exposed to 0.1 Gy (low dose) or 1.0 Gy (moderate dose) of whole-body gamma radiation and allowed to develop for 16 weeks. Liver mRNA profiles were then analyzed using the Affymetrix microarray platform, with validation by quantitative PCR. To be able to compare this to the acute response, 16-week old adults were exposed at the same doses and analyzed after 4 hours. We used 5 treatment groups: A=non-irradiated control, allowed to develop for 16 weeks; B=low-dose (0.1 Gy) irradiated, allowed to develop for 16 weeks; C=high-dose (1.0 Gy) irradiate, allowed to develop for 16 weeks; D=16 week old adults irradiated at low dose (0.1 Gy); E=16 week old adults irradiate at high dose (1.0 Gy)
2014-04-01 | E-GEOD-46026 | biostudies-arrayexpress
Project description:The selection of specific miRNAs by exosomes and their release from cultured melanocytes after exposure to solar UVR (UVA+UVB) have activities in inducing these cells into a premature senescence. In this analysis, human TaqMan microRNA array cards A+B were used to measure the relative expression of miRNAs in exosomes isolated from the irradiated and un-irradiated melanocytes.
2020-06-01 | GSE141619 | GEO
Project description:The purpose of this study is to collect prospective data for use as a comparator for future subsequent studies attempting to increase the efficacy or reduce the toxicity of gamma knife radiosurgery.
| 2156435 | ecrin-mdr-crc
Project description:Exosomes released by irradiated cells mediate radiation-induced bystander effect, which is manifested by DNA breaks detected in recipient cells, yet the specific mechanism responsible for generation of chromosome lesions remains unclear. In this study, naïve FaDu head and neck cancer cells were stimulated with exosomes released by irradiated (a single 2Gy dose) or mock-irradiated cells. Maximum accumulation of gamma H2A.X foci, a marker of DNA breaks, was detected after one hour of stimulation with exosomes from irradiated donors, the level of which was comparable to the one observed in directly irradiated cells (a weaker wave of the gamma H2A.X foci accumulation was also noted after 23 hours of stimulation). Exosomes from irradiated cells, but not from control ones, activated two stress-induced protein kinases: ATM and ATR. Noteworthy, while direct irradiation activated only ATM, both ATM and ATR were activated by two factors known to induce the replication stress: hydroxyurea and camptothecin (with subsequent phosphorylation of gamma H2A.X). One hour of stimulation with exosomes from irradiated cells suppressed DNA synthesis in recipient cells and resulted in the subsequent nuclear accumulation of RNA:DNA hybrids, which is an indicator of impaired replication. Interestingly, the abovementioned effects were observed before a substantial internalization of exosomes, which may suggest a receptor-mediated mechanism. After one hour of stimulation with exosomes from irradiated donors increased phosphorylation of several nuclear proteins was observed, including replication factors and regulators of heterochromatin remodeling, as well as components of multiple intracellular signaling pathways. Hence, we concluded that the bystander effect mediated by exosomes released from irradiated cells involves the replication stress in recipient cells.
2022-05-20 | PXD032143 | Pride
Project description:Samples of 3D skin, irradiated using LED light and compared with un-exposed control, regarding one- and four-days of incubation. Three groups were simulating acute exposure: 1h, 2h and 4 hours whereas the 3D skin samples irradiated for 1 hour over four sequential days were simulating repeated exposure, for both blue wavelength and the full visible spectrum of digital light.
2023-11-28 | GSE190106 | GEO
Project description:Gpr88 is an orphan G protein-coupled receptor highly expressed in the striatum, a region important for motor control and learning. We developed a mouse lacking this receptor (genotype Gpr88Cre/Cre) by replacing most of the open-reading-frame of the Gpr88 gene with Cre recombinase. When comparing the homozygous knockouts against wild-type mice (Gpr88+/+), we have observed that knockout mice are hyperactive, present motor deficits and impaired cue-based learning. However, the signaling pathways downstream of Gpr88 are unknown. To identify putative downstream factors we designed a microarray experiment to identify gene expression changes in the striata of animals lacking Gpr88.
2012-10-15 | E-MTAB-1282 | biostudies-arrayexpress
Project description:The molecular mechanisms underlying the changes in the nigrostriatal pathway in Parkinsonâs disease (PD) are not completely understood. Here we use microarrays and mass spectrometry to study the transcriptomic and proteomic changes in the striatum of two mouse models of PD induced by distinct neurotoxins, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and methamphetamine (METH). Transcripts and proteins were found with similar abundance changes in both models which may be involved in the pathophysiology of PD. GFAP transcript and protein levels were significantly up-regulated by both neurotoxins, confirming the known astrocytic response to these drugs. Other genes and proteins were idiosyncratic in their responses to the two toxins, suggesting specific toxicological responses. Comparing transcript and protein levels revealed that efficiently translated genes used more commonly occurring codons than inefficiently translated genes. Additionally, a potential role was found for miRNAs in translational control in the striatum. The results constitute one of the largest datasets integrating transcript and protein changes for these two neurotoxin models with many similar endpoint phenotypes but distinct pathologies. Using multiple toxins while examining proteins and transcripts can be an effective method of delineating the molecular pathology of neurodegenerative diseases. Experiment Overall Design: Parkinson's disease mouse models were created by injecting male C57BL/6J mice with either Methamphetamine or MPTP and were compared to saline injected controls. 3 biological replicates were examined for each condition yielding 9 total samples. Single channel Affymetrix mouse expression arrays 430_2 were used to analyze gene expression in the striata of each mouse.
2008-06-15 | E-GEOD-8030 | biostudies-arrayexpress