Project description:In the VILLIN-HA/CL4-TCR transgenic mouse model a population of CD8 T cells with suppressive capacities was indentified. For the molecular characterisation of CD8 regulatory T cells gene comparative expression profiles of naive, activated and regulatory CD8 T cells were performed. Three population of CD8 T cells are included in the analysis. 1. CD8 T cells 2. in vivo activated CD8 T cells 3. in vivo generated CD8 regulatory T cells
Project description:In the VILLIN-HA/CL4-TCR transgenic mouse model a population of CD8 T cells with suppressive capacities was indentified. For the molecular characterisation of CD8 regulatory T cells gene comparative expression profiles of naive, activated and regulatory CD8 T cells were performed.
Project description:Cellular binary fate decisions require the progeny to silence genes associated with the alternative fate. The major subsets of alpha:beta T cells have been extensively studied as a model system for fate decisions. While the transcription factor RUNX3 is required for the initiation of Cd4 silencing in CD8 T cell progenitors, it is not required to maintain the silencing of Cd4 and other helper T lineage genes. The other runt domain containing protein, RUNX1, silences Cd4 in an earlier T cell progenitor, but this silencing is reversed whereas the gene silencing after RUNX3 expression is not reverse. Therefore, we hypothesized that RUNX3 and not RUNX1 recruits other factors that maintains the silencing of helper T lineage genes in CD8 T cells. To this end, we performed a proteomics screen of RUNX1 and RUNX3 to determine candidate silencing factors.
