Project description:rs04-10_oxydative--stress - time course h2o2 treatment - Effect of an hydrogen peroxide treatment on gene regulation in arabidopsis cells - Arabidopsis cells (5 days after subculturing) were cultured at 21degreeC, 8 h photoperiod under agitation. H2O2 2.5 uM was added to cells 5 days after subculturing and 2 hours after the beginning of the light period. Treated and control cells were collected 15 min, 1 h, 5 h and 12 h after treatment. Keywords: treated vs untreated comparison
Project description:The phytopathological studies often deal with approaches involving the employment of high elicitor concentrations necessary to provoke responses that could be easily observed and quantified. We tested the range of gene expression changes for a low concentration chitosan elicitor treatment of seedlings, 0.001%, and we compared it with the higher one, 0.01%. We uncover both the quantitative and qualitative changes in gene expressions between the two treatments. Further on, we tested if the gene expression can be modified by the treatment with the defence-related CAPE peptide (10 uM). We discovered several interesting candidate genes specific for each of treatments, and combination of treatments, that would be worth of further more focused genetic studies.
