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Regulatory Divergence in Drosophila revealed by mRNA-seq

ABSTRACT: Regulatory Divergence in Drosophila revealed by mRNA-seq

OTHER RELATED OMICS DATASETS IN: GSE20421

PROVIDER: PRJNA125585 | ENA |

REPOSITORIES: ENA

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Regulatory Divergence in Drosophila revealed by mRNA-seq (trans-splicing)

Project description:Regulatory Divergence in Drosophila revealed by mRNA-seq (trans-splicing)

| PRJNA128387 | ENA

Regulatory Divergence in Drosophila revealed by mRNA-seq

Project description:These data were generated in a study to analyze the genetic causes of gene regulatory divergence. mRNA-seq libraies were prepared from poly(A)+ RNA prepared from whole F1 hybrids of D. melanogaster and D. sechellia (female 0-3d post eclosion, "D_mel_x_D_sec"). A mixed mRNA- seq library was prepared from D. melanogaster and D. sechellia poly(A) + RNA (mixed before library preparation, "D_mel_+_D_sec). Finally, separate mRNA-seq libraries were prepared from poly(A)+ RNA from each species ("D_mel" and "D_sec"). The results of this study showed that 78% of genes expressed in the two species are differentially expressed, and that cis- and trans-regulatory divergence affect 51% and 66% of expressed genes, respectively. Keywords: RNA-Seq

2010-02-20 | GSE20421 | GEO

Regulatory Divergence in Drosophila revealed by mRNA-seq (trans-splicing)

2010-07-20 | GSE22588 | GEO

Regulatory Divergence in Drosophila revealed by mRNA-seq (trans-splicing)

Project description:Deep Sequencing of mRNA from Drosophila melanogaster, Drosophila Sechellia, and their F1 hybrid. These data were generated in a study to analyze the extent and specificity of trans-splicing in Drosophila. mRNA-seq libraies were prepared from poly(A)+ RNA prepared from whole F1 hybrids of D. melanogaster and D. sechellia (female 0-3d post eclosion). Separate control libraries were prepared from D. melanogaster and D. sechellia total RNA (mixed before library preparation). The results of this study identified 80 novel trans-splicing events between homologous alleles of the same gene. Keywords: RNA-Seq Keywords: Expression profiling by high throughput sequencing There were 2 total samples in this study. Paired-end mRNA-seq was used to survey trans-splicing in F1 hybrids of D. melanogaster and D. sechellia. A mixed "control" library was used to assess the frequency of false-positive trans-splicing signal resulting from errors in reference genomes, high-throughput sequencing, and RT-PCR amplification based strand-switching.

2010-07-19 | E-GEOD-22588 | biostudies-arrayexpress

Spatially varying cis-regulatory divergence in Drosophila embryos elucidates cis-regulatory logic

Project description:Both cis- and trans-acting changes could accumulate and participate in complex interactions, so to isolate the cis-regulatory component of patterning evolution, we measured allele-specific spatial gene expression patterns in Drosophila melanogaster × D. simulans hybrid embryos. RNA-seq of cryosectioned slices revealed 55 genes with strong spatially-varying allele-specific expression, and several hundred more with weaker but significant spatial divergence. Combined with mathematical modeling and regulatory locus editing, we determine the SNP responsible for the allele-specific expression observed in the gene hunchback.

2017-08-04 | GSE102233 | GEO

Regulatory divergence in Drosophila revealed by mRNA-seq.

Project description: Not available

| S-EPMC2877578 | biostudies-literature

Drosophila Divergence 20091001

Project description:Drosophila Divergence 20091001

| PRJNA118209 | ENA

Spatially varying cis-regulatory divergence in Drosophila embryos elucidates cis-regulatory logic

Project description:Spatially varying cis-regulatory divergence in Drosophila embryos elucidates cis-regulatory logic

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Cis-regulatory divergence of eloF drives reproductive isolation in Drosophila

Project description:Cis-regulatory divergence of eloF drives reproductive isolation in Drosophila

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Cis-regulatory divergence of eloF drives reproductive isolation in Drosophila

Project description:The differential production of pheromones is a major barrier to mating between species in Drosophila. Individuals from morphologically similar sister species can produce different sets of cuticular hydrocarbons that allow potential mates to identify them as a suitable partner. In order to elucidate cis-regulatory mechanisms behind speciation, we looked for allele-specific expression in hydrocarbon-producing oenocytes from F1 hybrids of the sister species D. simulans and D. sechellia. By focusing on cis-regulatory changes specific to female oenocytes, we rapidly identified a small number of candidate genes. Oour RNA-seq approach proved to be far more efficient than QTL mapping in identifying candidate genes, and it can be used to pinpoint the genetic basis for a wide range of traits differing due to cis-regulatory divergence between any interfertile species.

2018-05-16 | GSE114478 | GEO

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