Hordeum vulgare
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA131735 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Analysis of barley grains/seedlings representing six well characterized and distinct germination stages over the course of seed germination and seedling growth.
2011-04-01 | GSE23595 | GEO
Project description:Analysis of barley grains/seedlings representing six well characterized and distinct germination stages over the course of seed germination and seedling growth. Three biological replications, six developmental stages.
2011-04-01 | E-GEOD-23595 | biostudies-arrayexpress
Project description:Geomagnetic field (GMF) has been present since the beginning of plant evolution. Recently, some researchers have focused their efforts on employing magnetic fields (MF) higher than GMF to improve the seed germination, growth and harvest of agriculturally important crop plants, as MFs are inexpensive and environment friendly technique. In this study, we have employed different treatments considering MF of 7 mT (milliTesla) for different time point of the exposure including 1, 3, and 6 h as treatment, following longest exposure for 5 consecutive days, 6h per day in barely seeds. The results showed a positive impact of MF on growth characteristics for 5 days old seedlings including seed germination rate, root and shoot length and biomass weight, however, significant effects observed in long exposure. Moreover, ~5 day’s delay of flowering in pretreated plants was observed. We have used a shotgun proteomics approach to identify changes in the protein signatures of root and shoot tissues under MF effects. In total, we identified 2896 proteins. Thirty eight proteins in shoot and 15 proteins in root showed significant changes under MF effect. Proteins involved in primary metabolic pathways were increased in contrast to the proteins with metal ion binding function, proteins contain iron ion in their structure and proteins involved in electron transfer chain were decreased significantly in treated tissues. The prevalent biological processes of the up-regulated proteins were carbohydrate metabolic process, oxidation-reduction process and cell redox homeostasis, while down regulated processes include translation and protein refolding. In general, shoot response was more significant to MF effect compared with root tissue leading to the identification of 41 shoot specific proteins. This study provides a comprehensive view of proteome regulation in response to MF during early stage of growth and development in barley.
2021-09-09 | PXD022079 | Pride
Project description:We hypothesized that the genome segments of cultivated barley should show certain similarity with its ancestral wild barley. Instead of whole genome sequences, we employed RNA-Seq to investigated the genomic origin of modern cultivated barley using some representative wild barley genotypes from the Near East and Tibet, and representative world-wide selections of cultivated barley.
2014-10-06 | E-MTAB-4221 | biostudies-arrayexpress
Project description:Purpose: The goal of this study was to investigate the mechanisms involved in the initiation and development of crown-roots (CRs) in barley and to estimate the role of cytokinins (CKs) in this process. Method: stranded libraries were obtained from RNA extracted from the stem base of 1 day-after-germination (DAG) and 10DAG-seedlings of wild-type (WT) and AtCKX-overexpressing barley lines (OE-CKX). OE-CKX lines have a reduced content of endogenous CKs and are characterized by a higher number of CRs. Libraries were deep sequenced on Illumina HighSeq platform. Each sample was investigated in three independent biological replicates. Results: Using a data analysis workflow optimized for barley, we identified more than 4000 transcripts differentially expressed in the stem base of 1DAG and 10DAG-seedlings. Expression as determined by RNA-seq was validated by real-time PCR. Our data were compared to the transcriptomic profiling obtained from rice and we were able to identify genes potentially involved in the initiation/development of CRs in barley. Also the use of the transgenic line with altered endogenous CK content allowed us to conclude about the role of CKs in the process. Conclusions: Our study represents the first analysis aiming to understand the initiation and development of CRs in barley.
2016-11-01 | GSE87737 | GEO
Project description:NILs containing five parental lines, three wild barley genotypes ssp. spontaneum: HID 4 (A), Iraq; HID 64 (B), Turkey; and HID 369 (C), Israel, one ssp. agriocrithon: HID 382(D)) and cv. Morex (ssp. vulgare, USA). Purpose: Variant calling to identifie markers associated with a awn length QTL on the distal part of chromosome 7HL
2017-03-08 | GSE85338 | GEO
Project description:Analysis of the barley leaf transcriptome under salinity stress using mRNA-Seq.
2012-12-31 | E-MTAB-4428 | biostudies-arrayexpress
Project description:In the present study, we investigated the transcriptome features during hulless barley grain development. Using Illumina paired-end RNA-Sequencing, we generated two data sets of the developing grain transcriptomes from two hulless barley landraces.
2014-06-19 | E-MTAB-4307 | biostudies-arrayexpress
Project description:Sequencing of five barley cultivars and one wild barley accession.
| PRJEB628 | ENA
Project description:Plant seeds prepare for germination already during seed maturation. We performed a detailed transcriptome analysis of barley grain maturation, desiccation and germination in two tissue fractions (endosperm/aleurone = e/a and embryo = em) using the Affymetrix barley1 chip. Keywords: time course
2007-12-20 | GSE9365 | GEO