Dataset Information

Magnetic field induced changes in the proteomic analysis in barley (Hordeum vulgare)

ABSTRACT: Geomagnetic field (GMF) has been present since the beginning of plant evolution. Recently, some researchers have focused their efforts on employing magnetic fields (MF) higher than GMF to improve the seed germination, growth and harvest of agriculturally important crop plants, as MFs are inexpensive and environment friendly technique. In this study, we have employed different treatments considering MF of 7 mT (milliTesla) for different time point of the exposure including 1, 3, and 6 h as treatment, following longest exposure for 5 consecutive days, 6h per day in barely seeds. The results showed a positive impact of MF on growth characteristics for 5 days old seedlings including seed germination rate, root and shoot length and biomass weight, however, significant effects observed in long exposure. Moreover, ~5 day’s delay of flowering in pretreated plants was observed. We have used a shotgun proteomics approach to identify changes in the protein signatures of root and shoot tissues under MF effects. In total, we identified 2896 proteins. Thirty eight proteins in shoot and 15 proteins in root showed significant changes under MF effect. Proteins involved in primary metabolic pathways were increased in contrast to the proteins with metal ion binding function, proteins contain iron ion in their structure and proteins involved in electron transfer chain were decreased significantly in treated tissues. The prevalent biological processes of the up-regulated proteins were carbohydrate metabolic process, oxidation-reduction process and cell redox homeostasis, while down regulated processes include translation and protein refolding. In general, shoot response was more significant to MF effect compared with root tissue leading to the identification of 41 shoot specific proteins. This study provides a comprehensive view of proteome regulation in response to MF during early stage of growth and development in barley.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Hordeum Vulgare (barley)

TISSUE(S): Root, Shoot

SUBMITTER: Palak Chaturvedi

LAB HEAD: Palak Chaturvedi

PROVIDER: PXD022079 | Pride | 2021-09-09

REPOSITORIES: Pride

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Publications

Magnetic Field Induced Changes in the Shoot and Root Proteome of Barley (<i>Hordeum vulgare</i> L.).

Shabrangy Azita A Ghatak Arindam A Zhang Shuang S Priller Alfred A Chaturvedi Palak P Weckwerth Wolfram W

Frontiers in plant science 20210223

The geomagnetic field (GMF) has been present since the beginning of plant evolution. Recently, some researchers have focused their efforts on employing magnetic fields (MFs) higher than GMF to improve the seed germination, growth, and harvest of agriculturally important crop plants, as the use of MFs is an inexpensive and environment-friendly technique. In this study, we have employed different treatments of MF at 7 mT (milliTesla) at different time points of exposure, including 1, 3, and 6 h. T ...[more]

PMID: 33708230

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Project description:Orchid Dendrobium Sonia (Sonia) has wide demand in floral market throughout the world due to its vibrant coloured flowers, flowering recurrence and dense inflorescence. Effects of different monochromatic light qualities (red, far-red, blue, white) on different developmental and growth responses such as seed gemination, shoot and root growth, chlorophyll and carotenoid accumulation were studied in Sonia. Asymbiotic seed germination was the highest under blue or white light (80%, p<0.001) and least under far-red (55%, p<0.001) suggesting that it could either be controlled by an exceptional novel function of Cryptochrome 1 or the blue wavelengths perceived by PHYA or PHYB in Sonia. All analyses were done in 6-months-old plants till 1 year of age. Shoot length increased significantly in white or red light (3.7-fold, 3.5-fold respectively) while was least under far-red (decreased by 1.6-fold) indicating the major regulatory role of PHYB for shoot growth. Root growth in red in Sonia was more similar to hypocotyl adventitious root (HAR) formation genetically considering PHYB transcript expression. We suggest that the root growth (HAR) under red in Sonia is partly attributed to PHYB, negatively regulated with HY5 transcription factor and positively associated with auxin biosynthesis and accumulation gene BABY BOOM (BBM2) and efflux carriers such as LIKE AUXIN RESISTANT 2 and 3 (LAX2, LAX3). Highest chlorophyll content under far-red and blue might be a hyper-response of SAR under FR light in Sonia. Taxonomic tree analysis finds Sonia closer to Phalenopsis and Dendrobium catenatum Lindl. The three phytochromes and one cryptochrome sequences which were similar to PHYA, B, C and CRY1 respectively. CRY1 was retrieved with one more isoform CRY1_X2, with all these sequences more similar to those of Oryza.sativa. Further study can clarify the indicating reason of a probable gene loss which is evident from the absence of any sequence similar to CRY2 in root RNA isolates of Dendrobium Sonia.

Dataset Information

Magnetic field induced changes in the proteomic analysis in barley (Hordeum vulgare)

Publications

Magnetic Field Induced Changes in the Shoot and Root Proteome of Barley (<i>Hordeum vulgare</i> L.).

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