Proteomic analysis of the promotive effect of plant-derived smoke on plant growth of chickpe

ABSTRACT: Plant-derived smoke plays a key role in seed germination and plant growth. To investigate the effect of plant-derived smoke on chickpea, a gel-free/label-free proteomic technique was used. Germination percentage, root/shoot length, and fresh biomass were increased in chickpea treated with 2000 ppm plant-derived smoke within 6 days. On treatment with 2000 ppm plant-derived smoke for 6 days, the abundance of 90 proteins including glycolysis-related proteins significantly changed in chickpea root. Proteins related to signaling and transport were increased; however, proteins related to protein metabolism, cell, and cell wall were decreased. The sucrose synthase for starch degradation was increased and total soluble sugar was induced in chickpea. Similarly, the proteins for nitrate pathway were increased and nitrate content was improved in chickpea. On the other hand, although secondary metabolism related proteins were decreased, flavonoid contents were increased in chickpea. Based on proteomic and immuno-blot analyses, proteins related to redox homeostasis were decreased and increased in root and shoot, relatively. Furthermore, fructose-bisphosphate aldolase was increased; while, phosphotransferase and phosphoglyceromutase were decreased in glycolysis. These results suggest that plant-derived smoke improves early stage of growth in chickpea with the balance of many cascades such as glycolysis, redox homeostasis, and secondary metabolism.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Cicer Arietinum (chickpea) (garbanzo)

TISSUE(S): Plant Cell, Root

SUBMITTER: Takafumi Itoh  

LAB HEAD: Setsuko Komatsu

PROVIDER: PXD007875 | Pride | 2018-02-12

REPOSITORIES: Pride