Proteomics

Dataset Information

57

Root isoprene formation alters lateral root development Maja Miloradovic van Doorn


ABSTRACT: Isoprene is a C5 volatile organic compound, which can protect aboveground plant tissue from abiotic stress such as short-term high temperatures and accumulation of reactive oxygen species (ROS). Here, we uncover new roles for isoprene in the plant belowground tissues. By analyzing Populus x canescens isoprene synthase (PcISPS) promoter reporter plants, we discovered PcISPS promoter activity in certain regions of the roots including the vascular tissue, the differentiation zone and the root cap. Treatment of roots with auxin or salt increased PcISPS promoter activity at these sites, especially in the developing lateral roots (LR). Transgenic, isoprene non-emitting poplar roots revealed an accumulation of O2 - in the same root regions where PcISPS promoter activity was localized. Absence of isoprene emission, moreover, increased the formation of LRs. Inhibition of NAD(P)H oxidase activity suppressed LR development, suggesting the involvement of ROS in this process. The analysis of the fine root proteome revealed a constitutive shift in the amount of several redox balance, signaling and development related proteins, such as superoxide dismutase, various peroxidases and linoleate 9S-lipoxygenase, in isoprene non-emitting poplar roots. Together our results indicate for isoprene a ROS-related function, eventually co-regulating the plant-internal signaling network and development processes in root tissue. This article is protected by copyright. All rights reserved.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Populus Trichocarpa (western Balsam Poplar) (populus Balsamifera Subsp. Trichocarpa)

TISSUE(S): Plant Cell, Root

DISEASE(S): Disease Free

SUBMITTER: Ann-Christine König  

LAB HEAD: Prof. Dr. Jörg-Peter Schnitzler

PROVIDER: PXD019789 | Pride | 2020-06-16

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
F062874.dat Other
F064685.dat Other
FRL7876A10MA3__010-9 FRL7876A10MA3-40-290664.raw Raw
FRL7876A11MA3__011-22 FRL7876A11MA3-40-290668.raw Raw
FRL7876A12MA3__012-8 FRL7876A12MA3-40-290672.raw Raw
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Publications

Root isoprene formation alters lateral root development.

Miloradovic van Doorn Maja M   Merl-Pham Juliane J   Ghirardo Andrea A   Fink Siegfried S   Polle Andrea A   Schnitzler Jörg-Peter JP   Rosenkranz Maaria M  

Plant, cell & environment 20200627 9


Isoprene is a C5 volatile organic compound, which can protect aboveground plant tissue from abiotic stress such as short-term high temperatures and accumulation of reactive oxygen species (ROS). Here, we uncover new roles for isoprene in the plant belowground tissues. By analysing Populus x canescens isoprene synthase (PcISPS) promoter reporter plants, we discovered PcISPS promoter activity in certain regions of the roots including the vascular tissue, the differentiation zone and the root cap.  ...[more]

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