Project description:This SuperSeries is composed of the following subset Series: GSE24282: CGH microarray analysis of human prostate adenocarcinoma and normal samples GSE24283: Deep transcriptional sequencing analysis of human prostate adenocarcinoma and reference samples Refer to individual Series
Project description:Prostate adenocarcinoma and matched adjacent normal samples were profiled for copy number with the Agilent 244A CGH Array to support a study of deep transcriptional sequencing on these samples.
Project description:Prostate adenocarcinoma and matched adjacent normal samples were profiled for copy number with the Agilent 244A CGH Array to support a study of deep transcriptional sequencing on these samples. Two-condition experiment: Prostate adenocarcinoma versus matched normal from three separate patients.
Project description:Prostate cancer cell lines grow in full serum under standard conditions were profiled on Agilent-014698 Human Genome CGH Microarray 105A. Digestion, labeling, hybridization and data analysis of genomic DNA were performed according to the Agilent Technologies (Santa Clara, CA) protocol version 6.0 for 105 K arrays. Ten prostate cancer cell lines were included in the profiling, 22RV1, C4-2B, CA-hpv-10, DU145, LAPC4, MDAPCa-2b, NCI660, PC3, VCaP, and WPE1-NB26. All cell lines were grown in accordance with the distributor's instructions. All samples except DU145 were hybridized once on 105k Agilent Human Genome CGH microarrays according to manufacturers instructions. Two replicate hybridizations were done for DU145 cell line. Human Adult Male Normal Genomic DNA (6 normal individuals, Promega, #G1471) was used as a reference in all hybridizations. DNA from cell line samples were labeled with Cy5 and DNA from reference were labeled with Cy3.
Project description:Genome-wide DNA methylation profiles were obtained from prostate adenocarcinoma samples (20) and corresponding cancer-unrelated normal prostate tissue (28) using Illumina HumanMethylation450 BeadChips. Approximately 485000 CpG sites were measured in every sample.