ABSTRACT: Identifying putative candidate genes and pathways involved in immune responses to porcine reproductive and respiratory syndrome virus (PRRSV) infection
Project description:This study investigated the immunological function of PRRSV Nsp1 by ectopic expression of PRRSV Nsp1 in 3D4/31 cell line. Identifying the functional role of PRRSV Nsp1 associated with host cell modulation may provide better knowledge about the pathogenesis of PRRS (Porcine reproductive and respiratory syndrome).
Project description:Transcription analysis of the porcine alveolar macrophage （PAM） response to co-infection of porcine reproductive and respiratory syndrome virus (PRRSV) and M. hyopneumoniae (Mhp)
Project description:We used the high-throughput sequencing and inhibitors to screen microRNAs that play the role in anti-porcine reproductive and respiratory syndrome virus (PRRSV) responses in porcine alveolar macrophages (PAMs).
Project description:Pathology and Protective Immune Response in Pigs Infected with Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) of High and Low Virulence
Project description:Microarray analysis of miRNAs involved in Porcine Alveolar Macrophages at the early stage of porcine reproductive and respiratory syndrome virus infection [LCS_miRPig_18]
Project description:Microarray analysis of miRNAs involved in Porcine Alveolar Macrophages at the early stage of porcine reproductive and respiratory syndrome virus infection [LCS_miRPig_17]
Project description:Porcine reproductive and respiratory syndrome virus (PRRSV) is a causative agent of an important infectious disease causing serious economic losses to swine industry called PRRS (porcine reproductive and respiratory syndrome). The clinical signs of this syndrome indcude respiratory disorders, abortions and variable mortality in piglets. To compare the virulence of highly diverse East European strains belonging to subtype 2 (Russian strain ILI and Belarusian strain BOR) and Danish strain from classical subtype 1 (DAN) the experimental study enrolling infection of piglets was performed. Gene expression profiles of peripheral blood mononuclear cells (PBMC) of piglets infected with three PRRSV strains vs control piglets were analysed by microarray analysis to gain insight into transcriptome changes after PRRSV infection.
Project description:Porcine reproductive and respiratory syndrome (PRRS), caused by PRRS virus (PRRSV), is the most economically important disease in pig populations. Lung damage is one major pathological condition following PRRSV infection, often leading to animal death. In vivo, PRRSV productive infection occurs predominately in alveolar macrophages of the lung. Here, transcriptome profiling of pulmonary alveolar macrophages (PAMs) from Tongcheng piglets pre- and post- infection of highly pathogenic PRRSV has been performed using porcine Affymetrix GeneChip.
