Homo sapiens
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ABSTRACT:
PROVIDER: PRJNA139587 | ENA |
REPOSITORIES: ENA
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Project description:Menthol, a naturally occurring cooling compound of peppermint oil, induces an anti-proliferative activity in androgen-independent prostate cancer (AIPC). Previously, we found that menthol affects PC-3 cells viability through activating JNK but the mechanism is not fully clear. We thus studied that dysregulated cell cycle progression of PC-3 cells to menthol. We performed microarray experiments to obtain a global view of how menthol affects prostate cancer biology in gene expression profile. Our study demonstrated that menthol induced G2/M arrest by dysregulating PLK1 in the AICP cell model.
2011-08-31 | GSE28241 | GEO
Project description:Menthol, a naturally occurring cooling compound of peppermint oil, induces an anti-proliferative activity in androgen-independent prostate cancer (AIPC). Previously, we found that menthol affects PC-3 cells viability through activating JNK but the mechanism is not fully clear. We thus studied that dysregulated cell cycle progression of PC-3 cells to menthol. We performed microarray experiments to obtain a global view of how menthol affects prostate cancer biology in gene expression profile. Our study demonstrated that menthol induced G2/M arrest by dysregulating PLK1 in the AICP cell model. Menthol was dissolved in ethanol as a vehicle at 0.1% working concentration and treated in PC-3 cells in triplicate Vehicle-treated PC-3 cells in triplicate were used as control.
2011-08-30 | E-GEOD-28241 | biostudies-arrayexpress
Project description:Several compounds were treated on the PC-3 cell line to investigate cell cycle regulation in prostate cancer through gene expression profile data. Our study demonstrated that several compounds induced cell cycle regulation in the CRPC (Castration-Resistant Prostate Cancer) cell model. Compounds (menthol, cineol, geraniol, icilin, linalool) were dissolved in ethanol as a vehicle at 0.1% working concentration and treated in PC-3 cells in triplicate. Vehicle-treated PC-3 cells in triplicate were used as a control.
2013-11-25 | E-GEOD-45567 | biostudies-arrayexpress
Project description:Prostate cancer (PCa) is the most common cancer in American men. The American Cancer Society’s estimates for prostate cancer in the United States for 2017 are estimated 161.360 new cases and 26,730 deaths from PCa. To study metastatic properties to bone, PC-3 cell line is mainly used classical human prostatic carcinoma cell line, established and characterized its tumorigenicity from a human prostatic adenocarcinoma metastatic to bone is reported. In addition, PC-3/nkR cell line, natural killer(NK) cells-resistant, was isolated from mammary tumor xenograft studies in mice from PC-3 was implanted to nude mice and fecund to be tumorigenic in the early 2000s. In this study, we investigated secreted proteins of the conditioned media of PC-3 and PC-3/nkR cell lines using comparative proteomics technology to identify the molecular mechanism related to metastatic processes related to PC-3/nkR. Our study showed PC-3/nkR cells are new highly migrated and NK cells-resistant cell-line compared to PC-3 cells, as novel highly malignant tumor cells to study mechanisms of PCa metastatic.
2019-08-19 | PXD008184 | Pride
Project description:We measured the effect of docetaxel treatment to three differentially responsive prostate cancer cell lines, LNCaP, DU145 and PC-3, based on a transcriptional time course response by microarray analysis. These cell lines represent both androgen independent (DU145 and PC-3) and androgen sensitive (LNCaP) cells
2016-07-21 | GSE83654 | GEO
Project description:High levels of GLI (GLI1 and GLI2) mRNA and GLI luciferase reporter activity were detected in the androgen independent prostate cancer cell lines DU145 and PC-3 compared to the androgen-dependent LNCaP prostate cancer cell line. Subsequently, we observed that ectopic GLI1 promoted hormone independence in LNCaP cells (LNCaP-GLI1). We compared the gene expression profile of LNCaP-pBP (empty vector), LNCaP-GLI1, DU145, and PC-3 cells globally as well as to identify GLI1-regulated genes that may contribute to hormone independence. RNA was harvested and analysed from LNCap-pBP (control/reference sample), LNCaP-GLI1, DU145 and PC-3 cells
2011-02-13 | E-GEOD-27231 | biostudies-arrayexpress
Project description:Gene expression profiling of LNCaP and PC-3 prostate cancer cell lines
| PRJNA109679 | ENA
Project description:High levels of GLI (GLI1 and GLI2) mRNA and GLI luciferase reporter activity were detected in the androgen independent prostate cancer cell lines DU145 and PC-3 compared to the androgen-dependent LNCaP prostate cancer cell line. Subsequently, we observed that ectopic GLI1 promoted hormone independence in LNCaP cells (LNCaP-GLI1). We compared the gene expression profile of LNCaP-pBP (empty vector), LNCaP-GLI1, DU145, and PC-3 cells globally as well as to identify GLI1-regulated genes that may contribute to hormone independence.
2011-02-13 | GSE27231 | GEO
Project description:Project Description: Extracellular vesicles were isolated from conditioned media of prostate cancer cell lines PC-3M and LNCaP. Proteomic profiling was conducted on EVs to identify differential protein cargoes.
2020-06-27 | PXD019441 | Pride
Project description:BACKGROUND. Human prostate cancer LNCaP and PC-3 cell lines have been extensively used as prostate cancer cell models to study prostate cancer progression and to develop therapeutic agents. Although LNCaP and PC-3 cells are generally assumed to represent early and late stages of prostate cancer development, respectively, there is limited information regarding comprehensive gene expression patterns between these two cells lines and relating these cells to prostate cancer progression based on their gene expression. METHODS. Comprehensive gene expression analysis was performed in LNCaP and PC-3 cells. Total RNA was isolated from cultured cells and hybridized to Illumina human Ref-8 version 3 BeadChips representing 24,526 transcripts. Bioinformatics approach was applied to identify genes, their functional roles and interaction networks that are unique in either LNCaP or PC-3 cells. RESULTS. We observed large differences in gene expression between LNCaP and PC-3 cells.Using robust statistical analysis and very high significance criteria to identify tractable number of genes 115 and 188 genes were identified uniquely expressed in LNCaP and PC-3 cells, respectively. Genes uniquely expressed in LNCaP cells contained UDP-glucosyltransferases as a signature for this cell line. This cell line demonstrated upregulation of various metabolic pathways on gene expression level. Talα/β, GATA-1 and c-Myc/Max were identified by in silico analysis as possible transcription factors regulating unique LNCaP genes. PC-3 cells were characterized by cytosceleton-related genes, keratins in particular. Several other well known genes (VEGFC, IL8, TGFβ2 and others) scattered throughout literature were identified and summarized in the discussion. CONCLUSIONS. This study demonstrated that LNCaP and PC-3 cells represent two distinct prostate cancer cell lineages. LNCaP cells retain many prostate cell specific properties, whereas PC-3 cells have acquired more aggressive bone-like characteristics following bone metastasis and show little resemblance to prostate cells. Microarray studies confirmed previously published results and provided more information between these two prostate cancer cell lines. Future studies need to consider their similarities and differences in gene expression between localized and metastasized prostate cancer.
2009-04-27 | GSE13131 | GEO