Schistosoma mansoni
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA171558 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Stem cells rejuvinate the schistosome host-parasite interface
| PRJNA305542 | ENA
Project description:Genetic and molecular basis of drug resistance and species-specific drug action in Schistosome parasites
| PRJNA225599 | ENA
Project description:Nitric oxide (NO) is a gaseous intercellular signaling molecule that also plays a role in host-parasite relations. NO acts rapidly, either via regulation of soluble guanylate cyclase, or by direct interactions with enzymes and other proteins, and has also been shown to have effects on gene expression. Here, we use SAGE (Serial Analysis of Gene Expression) to identify NO-responsive changes in gene expression in Schistosoma mansoni following a 3 hour exposure to sodium nitroprusside, an NO donor. Overall, these results indicate that NO does not rapidly induce large-scale changes in schistosome gene expression, but that expression of particular genes of interest appear to respond to NO. Keywords: Schistosoma, SAGE, NOS, nitric oxide, gene expression Adult S. mansoni perfused from infected Swiss-Webster female mice (obtained from the NIAID Schistosomiasis Resource Center) 42-49 days postinfection were maintained in culture (RPMI medium) overnight and then exposed for 3 hours to either 1 mM sodium nitroprusside (SNP), a well-characterized NO donor, or to RPMI alone. Worms remained viable and motile following treatment. Total RNA was extracted with Trizol (Invitrogen) and treated with DNAse 1 (Ambion) to remove contaminating genomic DNA, and Long-SAGE libraries constructed.
2007-07-04 | E-GEOD-8366 | biostudies-arrayexpress
Project description:A kinetic model of S. mansoni glycolysis in which we can vary the allosteric regulation on lactate dehydrogenase (LDH). Because not enough kinetic data are available for all the schistosome enzymes to construct a complete schistosome glycolysis model de novo, we here adjusted the well-established glycolysis model of the eukaryotic microbe Saccharomyces cerevisiae (Teusink et al., 2000; van Eunen et al., 2012; van Heerden et al., 2014). We removed reactions from the yeast model that are not used in schistosomes and added glycogen metabolism, a Krebs cycle, respiration and an allosterically regulated LDH that was parameterized on multiple in vitro kinetic data sets for schistosome LDH. Additional files are published on Zenodo: https://zenodo.org/records/10401097
2024-04-04 | MODEL2208290002 | BioModels
Project description:Suppression subtractive hybridization(SSH) libraries of Schistosoma japanicum female and male worms were constructed by using Clontech PCR-selectTM cDNA subtraction kit. S.japonicum cDNA microarrays were fabricated using female and male cDNA clones originating from SSH libraries. female-associated and Male-associated differentially expressed gene clones were obtained, Analysis of gender-associated differentially expressed genes helps to determine which genes are important to sexual maturation of schistosome and better understand schistosome biology and host-parasite relationship, facilitate the discovery of novel gene products that could represent targets for the development of new drugs and vaccines to control chitosomiasis. Keywords: gender-associated
2012-03-05 | GSE3641 | GEO
Project description:Protein variation in blood-dwelling schistosome worms generated by differential splicing of micro-exon gene transcripts. The infective schistosome cercaria develops from an undifferentiated germ ball within the daughter sporocyst in the molluscan host, during which considerable morphological development and synthesis of proteins essential for infection occurs. The free-living, non-feeding cercaria is notable for its swimming and host location behaviour. On contact with host skin it rapidly penetrates, replaces its tegument surface membranes, and begins body remodelling, before crossing the dermis to exit the skin via a blood vessel. Such a ‘violent’ transition from snail to fresh water to mammalian host should be accompanied by remarkable changes in the patterns of gene expression. All gene models from version E of the S. mansoni genome (www.GeneDB.org) were incorporated into a 350K feature Roche-NimbleGen, high-density oligonucleotide array. From a map-ordered list, every 13th 50mer was chosen as a probe. Double-stranded cDNA from three biological replicates each of germ balls, cercariae, and day 3 schistosomula was hybridised to the array without amplification. Statistical analysis was carried out using programmes from the Bioconductor suite (Gentleman et al. 2004 Genome Biol 5(10):R80). More than 1000 loci were shown to be differentially expressed in each of the comparisons between the three life cycle stages. Gene ontology (GO) analysis was then carried out to discover the categories enriched in each stage. In addition custom categories based on biological function (e.g. stress response) or parasite tissue (e.g. tegument) were analysed.
2010-05-29 | GSE22037 | GEO
Project description:Intestinal Schistosomiasis, caused by the helminth Schistosoma mansoni, results in fibroproliferative disease triggered by liver trapped parasite eggs. Characteristic of the infection is a chronic course of disease with progressive periportal fibrosis, spleno- and hepatomegaly, and portal hypertension. Despite significant progress in understanding the complex immunological processes involved in the host-pathogen relationship, there is currently no effective vaccine against the infection nor antifibrotic drugs, increasing the pressure to find new targets for schistosome drugs and vaccine candidates. We have recently reported that infection with male schistosomes can attenuate reinfections, while female schistosomes appear to weaken the host immune system. To decipher the underlying mechanisms of differential immunogenicity of female and male worms, we performed a comparative transcriptomic analysis of splenic tissue from unisexually and bisexually infected mice. We identified a total of 1293, 512 and 4062 genes, respectively, that were differentially expressed in the group of male-only (M), female-only (F) or bisexually (MF) infected mice compared to naive controls. Bisexual infection and infection with male worms showed the marked effects on host immune response, erythrocyte homeostasis, lipoprotein metabolism and cell cycle processes compared to the infection with female worms. Furthermore, we could show that both worm sexes trigger immune responses in an egg-independent manner, with a non-polarized Th1 and Th2 response, with female worms having a lower regulatory influence than males.
2022-06-16 | GSE197804 | GEO
Project description:Schistosomula exposed to HDAC inhibitor (Trichostatin A) [24hrs]
| PRJNA325344 | ENA
Project description:Schistosomula exposed to HDAC inhibitor (Trichostatin A) [48hrs]
| PRJNA325340 | ENA
Project description:Schistosomula exposed to HDAC inhibitor (Trichostatin A) [12hrs]
| PRJNA325345 | ENA