Project description:Salt stress is one of the most severe environmental conditions which cause huge losses in crop production worldwide. We identified an essential regulator of salt stress RSA3 and used the Affymetrix whole-genome arrays to study the effect of rsa3-1 mutation on global gene expression under salt stress. A set of genes differentially expressed in rsa3-1 under salt stress are identified. Six-day-old seedlings of Arabidopsis thaliana wild type (Columbia gl1 expressing RD29A::LUC transgene) and rsa3-1 mutant seedlings subjected to salt stress at 120 mM NaCl for 24 h were used for total RNA extraction and hybridizations with Affymetrix ATH1 GeneChips. There are two biological replicates per genotype.
Project description:Salt stress is one of the most severe environmental conditions which cause huge losses in crop production worldwide. We identified an essential regulator of salt stress RSA3 and used the Affymetrix whole-genome arrays to study the effect of rsa3-1 mutation on global gene expression under salt stress. A set of genes differentially expressed in rsa3-1 under salt stress are identified.
Project description:Arabidopsis thaliana is a glycophyte with a low salt tolerance, while Eutrema is a halophyte with a very high salt tolerance. To elucidate the transcriptional basis of this difference, we performed hydroponis culture experiments where we grew plants under control conditions (25 mM NaCl) or under salt stress (200 mM NaCl for both species, 500 mM for Eutrema). Salt concentration was increased for the stress treatments by increments of 50 mM per day (25 mM on the first day). Plants were grown at the final NaCl concentration for an additional week, when rosettes were harvested for RNA isolation.Expression patterns were compared between treatments and between species.
Project description:CuZn-superoxide dismutase (CuZn-SOD) and ascorbate peroxidase (APX) constitute first line of defence against oxidative stress. In the present study, PaSOD and RaAPX genes from Potentilla atrosanguinea and Rheum australe, respectively were overexpressed individually as well as in combination in Arabidopsis thaliana. We performed RNA-seq analysis of wild type and transgenic Arabidopsis thaliana overexpressing CuZn-SOD, APX and CuZn-SOD + APX under control and salt stress
Project description:To analyze global changes in AS under salt stress, we obtained high-coverage (~200 times) RNA sequencing data from Arabidopsis thaliana seedlings that were treated with different concentrations of NaCl. Our study provided a comprehensive view of AS under salt stress and revealed novel insights into the potential roles of AS in plant response to salt stress.
Project description:The overall objective was to document transcriptomic changes in the guard cells of Arabidopsis thaliana under short (1x) - and long-term (3x) saline growth conditions. Guard cells of Arabidopsis responded also to the intensity of the salt management by the microarray datasets clearly clustering in (-) salt, 1x salt and 3x salt. Similarly, more GO terms were significantly enriched in differentially expressed guard cell genes of 1 x salt than 3x salt treated plants.
