Project description:Genome wide map of heterochromatin state in fission yeast Schizosaccharomyces pombe via 4 different strains Examination of a single histone modification in 4 different fission yeast strains
Project description:Here, we report the high-throughput profiling of histone modification (H3K9me2) in fission yeast Schizosaccharomyces pombe. We generated genome-wide H3K9me2 maps of fission yeast mutants in swo1-26 (temperature sensitive, ts) cells at 25℃ and 37℃. We find that H3K9me2 enrichment at heterochromatin regions, especially at the mating-type locus and subtelomeres, is compromised, suggesting heterochromatin assembly defects.
Project description:We report the high-throughput profiling of H3K9me2 in fission yeast Schizosaccharomyces pombe. By obtaining 1-10 ng immunoprecipitated DNA, we generated genome-wide H3K9me2 maps in single deletions of the selected chromatin modulating genes, histone H3 mutants and ccp1Δepe1Δ double mutant in fission yeast. We find that the subtelomeric heterochromatin distribution is highly variable under different genetic perturbations and small heterochromatin islands are formed at clr4 and clr2 loci in ccp1Δepe1Δ.
Project description:We report the high-throughput profiling of histone variant CNEP-A/Cnp1 in fission yeast Schizosaccharomyces pombe. By obtaining 1-10 ng immunoprecipitated DNA, we generated genome-wide CENP-A/Cnp1 maps of the meiotic progeny from the genetic crossing of mhf2∆ (cen1_inactive) × clr4∆ (cen1_active) in fission yeast. We find that heterochromatin is not required for the induction of centromere inactivation.
Project description:We report the high-throughput profiling of histone variant CNEP-A/Cnp1 in fission yeast Schizosaccharomyces pombe. By obtaining 1-10 ng immunoprecipitated DNA, we generated genome-wide CENP-A/Cnp1 maps of clr4∆ and the transformants from the deletion of clr4 in mhf2∆ (cen2_inactive) or mhf2+ (cen1_inactive) in fission yeast. We find that heterochromatin is not required for the induction of centromere inactivation in mhf2∆ and the maintenance of neocentromere in mhf2+.
