Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Escherichia coli str. K-12 substr. MG1655

ABSTRACT: High resolution view of bacteriophage lambda gene expression by ribosome profiling

PROVIDER: PRJNA205933 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

High resolution view of bacteriophage lambda gene expression by ribosome profiling

2013-07-01 | GSE47509 | GEO

RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655

Project description:Expression profiles of wild-type and SgrR mutant E. coli strains under aMG and 2-DG-induced stress. Expression profiles of E. coli overexpressing SgrS sRNA.

2013-03-26 | GSE45443 | GEO

RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655

Project description:Expression profiles of wild-type and SgrR mutant E. coli strains under aMG and 2-DG-induced stress. Expression profiles of E. coli overexpressing SgrS sRNA. Illumina RNA-Seq of total RNA extracted from wild-type, SgrR/SgrS mutant and SgrS overexpressing E. coli strains grown in different conditions.

2013-03-26 | E-GEOD-45443 | biostudies-arrayexpress

Escherichia coli str. K-12 substr. MG1655

Project description:High Precision Analysis of Translational Pausing in Bacteria Lacking EFP by Ribosome Profiling

| PRJNA271104 | ENA

Escherichia coli str. K-12 substr. MG1655

Project description:High-resolution mapping of genome position-dependent transcriptional propensity in Escherichia coli

| PRJNA473168 | ENA

High resolution view of bacteriophage lambda gene expression by ribosome profiling

Project description:Bacteriophage lambda is one of the most extensively studied organisms, and has been a primary model for understanding basic modes of genetic regulation. Here we examine the progress of lambda gene expression during phage development by ribosome profiling, and thereby provide a very high resolution view of lambda gene expression. The known genes are expressed in a predictable fashion, authenticating the analysis. But many previously unappreciated potential open reading frames become apparent in the expression analysis, revealing an unexpected complexity in the pattern of lambda gene function. We chose temperature induction of the classic cI857 repressor mutation in a lysogen of E. coli MG1655 in order to synchronize the lytic process, sampling the lysogen and control non-lysogen both before and 2, 5, 10, and 20 minutes after shifting the temperature from 32M-BM-0 to 42M-BM-0. The last sample time was chosen to be before any significant cell lysis, but during the later stages of lytic gene expression. Total protected nucleotides within open reading frames were summed to determine the density of translation of each reading frame. We take this number to indicate the overall rate of translation, although obviously this assumes that pauses in translation do not excessively affect the overall rate. Since the expression level is not normalized for the copy number of the replicating phage DNA, it thus encompasses both the effect of DNA template availability on mRNA synthesis and the efficiency of utilization of messengers by ribosomes.

2013-07-01 | E-GEOD-47509 | biostudies-arrayexpress

YAMAT-seq of mature tRNA pools in the bacterium Escherichia coli str. K-12 substr. MG1655 and derivatives

Project description:Mature tRNA pools were measured using an adaptation of YAMAT-seq (Shigematsu et al., 2017; doi:10.1093/nar/gkx005 ) and further described in (Ayan et al., 2020; doi:10.7554/eLife.57947) in 10 strain-medium combinations (all strains dervied from the model bacterium E. coli MG1655). The aim of the experiment was to investigate the effect of reducing tRNA gene copy number on mature tRNA pools in rich and poor media.

2022-12-02 | GSE198606 | GEO

Escherichia coli str. K-12 substr. MG1655

Project description:A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function.

| PRJNA358097 | ENA

Escherichia coli str. K-12 substr. MG1655

Project description:Clarifying the translational pausing landscape in bacteria by ribosome profiling

| PRJNA295296 | ENA

Escherichia coli str. K-12 substr. MG1655

Project description:Ribosome profiling of E. coli K-12 MG1655 MOPS rich media with 0.2% glucose

| PRJNA269272 | ENA