Project description:Mesorhizobium huakuii 7653R is an α-proteobacterium that occurs either in a nitrogen-fixing symbiosis with its host plant, A. sinicus, or free-living in the soil. Investigation of whole genome gene expression level changes in Bacteroids compared to the free-living cells. Understand how M. huakuii 7653R responds to alterations in its environment and to the physiological changes that occur during bacteroid differentiation.
Project description:Mesorhizobium huakuii 7653R is an α-proteobacterium that occurs either in a nitrogen-fixing symbiosis with its host plant, A. sinicus, or free-living in the soil. We performed RNA-Seq on free-living cells grown in rich media and in bacteroids isolated from root nodules to understand how M. huakuii 7653R responds to alterations in its environment and to the physiological changes that occur during bacteroid differentiation. Understand how M. huakuii 7653R responds to alterations in its environment and to the physiological changes that occur during bacteroid differentiation.
Project description:Mesorhizobium huakuii 7653R is an M-NM-1-proteobacterium that occurs either in a nitrogen-fixing symbiosis with its host plant, A. sinicus, or free-living in the soil. Investigation of whole genome gene expression level changes in Bacteroids compared to the free-living cells. Understand how M. huakuii 7653R responds to alterations in its environment and to the physiological changes that occur during bacteroid differentiation. Examination of mRNA levels in free-living cells and bacteroids at 32 days postinoculation
Project description:Mesorhizobium huakuii 7653R is an M-NM-1-proteobacterium that occurs either in a nitrogen-fixing symbiosis with its host plant, A. sinicus, or free-living in the soil. We performed RNA-Seq on free-living cells grown in rich media and in bacteroids isolated from root nodules to understand how M. huakuii 7653R responds to alterations in its environment and to the physiological changes that occur during bacteroid differentiation. Understand how M. huakuii 7653R responds to alterations in its environment and to the physiological changes that occur during bacteroid differentiation. Examination of mRNA levels in free-living cells and bacteroids at 32 days postinoculation
Project description:Microarray Analysis Reveal Global Differential Transcritomes of Mesorhizobium huakuii 7653R between Bacteroids and Free-living Cells
Project description:Hydrogenases catalyze the simple yet importantredox reaction between protons and electrons and H2, thus, mediate interactions between microorganisms and their chemical environment. A mutation in M. huakuii hypE gene was generated by homologous recombination. The hypE mutant grew a little faster than its parental 7653R. Real-time quantitative PCR showed that the hypE gene expression is significantly upregulated in all the detected stages of nodule development. The wild type contained normal spherical shape of the nodules, while the hypE mutant elicited small-size nodules. Plants inoculated with mutant HKhypE showed a significantly more than 47% decrease in acetylene reduction activity compared to the wild-type 7653R. Proteomics analysis showed seven proteins were up-regulated and eighty-two proteins were down-regulated in hypE triple mutant bacteroids.