Project description:Gene expression assessment in blood from male Cynomolgus monkey after endotoxin challenge followed by intravenous infusion with peptide control (LR12-scrambled) or LR12. The samples were analyzed at different time-points: 0, 2, 4 and 8 hours
Project description:In order to develop new safe and potent therapeutics, insights into the mechanisms underlying diabetes mellitus are urgently needed. We used proteomics profiling of the liver tissue from Macaca fascicularis with spontaneously occurred diabetes mellitus at their middle age and including two groups of the monkeys fed with the same food and high-fat and high-sugar diet for comparison.We hoped to find something new for diabetes mellitus treatment.
Project description:Next generation sequencing reveals a controlled immune response to Zaire Ebola virus challenge in cynomolgus macaques immunized with VSVΔG/ZEBOVgp
Project description:A temporal study of gene expression in peripheral blood leukocytes (PBLs) from a Mycobacterium tuberculosis primary, temporal pulmonary challenge model Macaca fascicularis has been conducted. PBL samples were taken prior to challenge and at one, two, four an six weeks post-challenge and labelled, purified RNAs hybridised to Operon Human Genome AROS V4.0 slides. Data analyses revealed a large number of differentially regulated gene entities, which exhibited temporal profiles of expression across the time course study. Further data refinements identified key groups of markers showing specific group-specific expression patterns, with a substantial reprogramming event evident at the four to six week interval. Select statistically-significant gene entities from this study and other immune and apoptotic markers were validated using qPCR. These confirmed many of the observations elucidated using microarray hybridisation. The results showed evidence of a step-change from an ‘early’ perhaps FOS-driven response, to a predominantly type I interferon-driven response, with coincident reduction of expression of other markers i.e. loss of T-cell-associate marker expression in responsive animals and elevation of markers which may be associated with a myeloid suppressor cell phenotype e.g. CD163. The animals in the study were of different lineages, Chinese and Mauritian, which showed clear evidence of differing susceptibilities to Tuberculosis challenge. Further analyses determined a number of key differences in response profiles between the groups, particularly in expression of T-cell and apoptotic makers, among others. These have provided interesting insights into innate susceptibility phenotypes. Using a combination of parametric and non-parametric artificial neural network analyses we have identified key genes and regulatory pathways which may be important in earl and adaptive responses to TB.
